FCM (Flow Cytometry)
(Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 ug/10^6 cells of Anti-RIGI antibody (ABM40B5). Green represent isotype control and red represent Anti-RIG I antibody (AAA14865). Goat anti-mouse PE conjugate was used as secondary.)
Mouse anti-Human RIG-I Monoclonal Antibody | anti-RIG-I antibody
RIG-I Monoclonal Antibody
Gene Names
DDX58; RIGI; RIG-I; RLR-1; SGMRT2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Protein G Chromatography
Synonyms
RIG-I, Antibody; RIG-I Monoclonal Antibody; anti-RIG-I antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1, Kappa
Clone Number
ABM40B5
Purity/Purification
Protein G Chromatography
Form/Format
Purified; 25ug in 50ul/100ug in 200ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-RIG-I antibody
Immunohistochemistry (IHC),Flow Cytometry (FACS), Western blot (WB).
Application Notes
Western blot analysis: 2-4ug/ml
Immunohistochemical analysis: 5ug/ml
Flowcytometric analysis: 0.5-1ug/10^6 cells
Immunohistochemical analysis: 5ug/ml
Flowcytometric analysis: 0.5-1ug/10^6 cells
Immunogen
A partial length recombinant human RIG-I protein (amino acids 1-220) was used as the immunogen for this antibody.
Preparation and Storage
Store antibody at 4°C, stable for 6 months. For long-term storage, store at -20° C. Avoid repeated freeze and thaw cycles.
FCM (Flow Cytometry)
(Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 ug/10^6 cells of Anti-RIGI antibody (ABM40B5). Green represent isotype control and red represent Anti-RIG I antibody (AAA14865). Goat anti-mouse PE conjugate was used as secondary.)
FCM (Flow Cytometry)
(Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 ug/10^6 cells of Anti-RIGI antibody (ABM40B5). Green represent isotype control and red represent Anti-RIG I antibody (AAA14865). Goat anti-mouse PE conjugate was used as secondary.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of RIG I in normal human prostate tissue using RIG I antibody (Clone: ABM40B5) at 5 ug/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of RIG I in normal human prostate tissue using RIG I antibody (Clone: ABM40B5) at 5 ug/ml.)
WB (Western Blot)
(Expression analysis of RIGI. Anti- RIGI antibody (Clone:ABM40B5) was used at 2 µg/ml on K562 Lysates.)
WB (Western Blot)
(Expression analysis of RIGI. Anti- RIGI antibody (Clone:ABM40B5) was used at 2 µg/ml on K562 Lysates.)
Related Product Information for anti-RIG-I antibody
RIG-I or Probable ATP-dependent RNA helicase DDX58 or simply DDX58 is a RNA helicase enzyme with two isoforms (106 and 101 kDa). RIG-I belongs to the DEAD/H box family and is involved in several cellular responses including RNA binding and alteration of RNA secondary structure. RIG-1 acts as sensor of viral nucleic acids thus activating a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Generally it remains as a monomer but upon activation by viral dsRNA it homomultimerizes and interacts with mitochondria antiviral signaling protein (MAVS/IPS1) which activating a cascade of reactions to produce specific interferon. RIG-1 is mostly expressed in vascular smooth cells.
Product Categories/Family for anti-RIG-I antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
probable ATP-dependent RNA helicase DDX58
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
NCBI Official Symbol
DDX58
NCBI Official Synonym Symbols
RIGI; RIG-I; RLR-1; SGMRT2
NCBI Protein Information
probable ATP-dependent RNA helicase DDX58; DEAD box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; RNA helicase RIG-I; retinoic acid-inducible gene 1 protein; retinoic acid-inducible gene I protein
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX58
UniProt Gene Name
DDX58
UniProt Synonym Gene Names
RLR-1; RIG-1; RIG-I
UniProt Entry Name
DDX58_HUMAN
Similar Products
Product Notes
The RIG-I ddx58 (Catalog #AAA14865) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RIG-I Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RIG-I can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC),Flow Cytometry (FACS), Western blot (WB). Western blot analysis: 2-4ug/ml Immunohistochemical analysis: 5ug/ml Flowcytometric analysis: 0.5-1ug/10^6 cells. Researchers should empirically determine the suitability of the RIG-I ddx58 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIG-I, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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