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FCM (Flow Cytometry)
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Mouse anti-Human RING1 Monoclonal Antibody | anti-RING1 antibody

RING1 Antibody

Gene Names
RING1; RNF1; RING1A
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
RING1, Antibody; RING1 Antibody; Ring1A antibody; E3 ubiquitin-protein ligase RING1 antibody; Polycomb complex protein RING1 antibody; Really interesting new gene 1 protein antibody; RING finger protein 1 antibody; Ring1 antibody; RING1_HUMAN antibody; RING1A antibody; Rnf1 antibody; Transcription repressor Ring1A antibody; anti-RING1 antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
F4-C12
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RING1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:100-1:200
FC/FACS: 1:100-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of Hela cells with Ring1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of Ring1 on different cell lysate using anti-Ring1 antibody at 1/1, 000 dilution. Positive control�� Line1: MOLT-4 Line2: LNCaP Line3: Hela Line4: HEK-293 Line5: Jurkat)

WB (Western Blot)

(Western blot analysis of Ring1 on HEK293 (1) and Ring1-hIgGFc transfected HEK293 (2) cell lysate using anti-Ring1 antibody at 1/1, 000 dilution.)

WB (Western Blot)

(Western blot analysis of Ring1 on human Ring1 recombinant protein using anti-Ring1 antibody at 1/1, 000 dilution.)

Related Product Information for anti-RING1 antibody
In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci during embryonic development. The human PcG homologues form two distinct multimeric protein complexes, the EED/EZH protein complex and the HPC/HPH protein complex, which have mutually exclusive expression patterns. The HPC/HPH PcG complex contains the human polycomb 2 (HPC2), human polyhomeotic (HPH), Bmi-1 and RING1 proteins. The human RING1 gene, which is proximal to the major histocompatibility complex region on chromosome six, encodes for a protein that contains a RING finger motif, a zinc-binding domain found in many regulatory proteins, but unlike the other human PcG genes, RING1 displays no homology to known Drosophila PcG genes. RING1 strongly represses En-2, the mammalian homolog of the Drosophila engrailed gene, and when overexpressed, it mediates an increase in the expression of proto-oncogenes, such as c-Jun and c-fos. Also, loss of RING1 and Bmi-1 expression correlates with the differentiation of B cells, which suggests a role for RING1 in germinal center development.
Product Categories/Family for anti-RING1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
406
NCBI Official Full Name
E3 ubiquitin-protein ligase RING1
NCBI Official Synonym Full Names
ring finger protein 1
NCBI Official Symbol
RING1
NCBI Official Synonym Symbols
RNF1; RING1A
NCBI Protein Information
E3 ubiquitin-protein ligase RING1; polycomb complex protein RING1; really interesting new gene 1 protein
UniProt Protein Name
E3 ubiquitin-protein ligase RING1
UniProt Gene Name
RING1
UniProt Synonym Gene Names
RNF1
UniProt Entry Name
RING1_HUMAN

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Product Notes

The RING1 ring1 (Catalog #AAA29906) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RING1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RING1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:100-1:200 FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the RING1 ring1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RING1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.