Rabbit anti-Mouse RIP3 (RIPK3, Receptor-interacting Serine/Threonine-protein Kinase 3, RIP-like Protein Kinase 3, Receptor-interacting Protein 3, RIP-3) Monoclonal Antibody | anti-RIP3 antibody

RIP3 (RIPK3, Receptor-interacting Serine/Threonine-protein Kinase 3, RIP-like Protein Kinase 3, Receptor-interacting Protein 3, RIP-3)[KO Validated]

Cat. Num. AAA26908

• Gene Names: Ripk3; Rip3; AW107945; 2610528K09Rik
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Purity: Purified
• Synonyms: RIP3 (RIPK3, Receptor-interacting Serine/Threonine-protein Kinase 3, RIP-like Protein Kinase 3, Receptor-interacting Protein 3, RIP-3), Antibody; RIP3 (RIPK3, Receptor-interacting Serine/Threonine-protein Kinase 3, RIP-like Protein Kinase 3, Receptor-interacting Protein 3, RIP-3)[KO Validated]; RIP3; anti-RIP3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: D4G2A
• Specificity: Recognizes endogenous levels of total mouse RIP3.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-RIP3 antibody: Flow Cytometry (FC/FACS), Immunochemistry (IC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB), Knockout (KO) Validated
• Application Notes: Immunofluorescence (IF-IC): 1:800; Flow Cytometry: 1:200; Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:100
• Immunogen: Synthetic peptide corresponding to residues surrounding Val370 of mouse RIP3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

FCM (Flow Cytometry)

(Flow cytometric analysis of Neuro2A cells (blue) and L-929 cells (green) using RIPK3 mouse Rabbit mAb. Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of L-929 (left) and Neuro-2a (right) cells using AAA26908 (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).)

WB (Western Blot)

(Immunoprecipitation of RIP3 from L-929 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit mAb IgG Isotype Control, and lane 3 is AAA26908. Western Blot analysis was performed using AAA26908. A conformation-specific secondary antibody was used to avoid cross reactivity with IgG.)

WB (Western Blot)

(Western Blot analysis of extracts from wild-type (+) or RIP3 knockout (-) mouse spleen using AAA26908 (upper) or beta-Actin Rabbit mAb (lower). Data were kindly provided by Dr. Junying Yuan, Harvard Medical School, Boston MA.)

WB (Western Blot)

(Western Blot analysis of extracts from various cell lines using AAA26908 (upper) or B-Actin Rabbit mAb (lower).)

WB (Western Blot)

(Western Blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length mouse RIP3 (mRIP3; +) using AAA26908.)

Related Product Information for anti-RIP3 antibody

The receptor-interacting protein (RIP) family of serine-threonine kinases (RIP, RIP2, RIP3, and RIP4) are important regulators of cellular stress that trigger pro-survival and inflammatory responses through the activation of NF-kB, as well as pro-apoptotic pathways. In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and recruitment to TNF-R1 through interaction with TRADD. RIP-deficient cells show a failure in TNF-mediated NF-gammaB activation, making the cells more sensitive to apoptosis. RIP also interacts with TNF- receptor-associated factors (TRAFs) and can recruit IKKs to the TNF-R1 signaling complex via interaction with NEMO, leading to IgB phosphorylation and degradation. Overexpression of RIP induces both NF-gB activation and apoptosis. Caspase-8-dependent cleavage of the RIP death domain can trigger the apoptotic activity of RIP.||Receptor-interacting protein 3 (RIP3) was originally found to interact with RIP and the TNF receptor complex to induce apoptosis and activation of NF-kB. It has subsequently been shown that the association between RIP and RIP3 is a key component of a signaling pathway that results in programmed necrosis (necroptosis), a necrotic-like cell death induced by TNF in the presence of caspase inhibitors. RIP3 is phosphorylated at Ser227 and targets the phosphorylation

Product Categories/Family for anti-RIP3 antibody

Antibodies-Primary; Antibodies-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 6063101
• NCBI GeneID: 56532
• UniProt Accession #: Q9QZL0; Q3U3Z9; Q8K2Y2; G3X8V8
• NCBI Official Full Name: receptor interacting protein 3
• NCBI Official Synonym Full Names: receptor-interacting serine-threonine kinase 3
• NCBI Official Symbol: Ripk3
• NCBI Official Synonym Symbols: Rip3; AW107945; 2610528K09Rik
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 3; RIP-3; mRIP3; RIP-like protein kinase 3; receptor interacting protein 3; receptor-interacting protein 3
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 3
• UniProt Gene Name: Ripk3
• UniProt Synonym Gene Names: Rip3; RIP-3; mRIP3
• UniProt Entry Name: RIPK3_MOUSE