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FCM (Flow Cytometry)
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Rabbit SF3B3 Monoclonal Antibody | anti-SF3B3 antibody

SF3B3 Antibody

Gene Names
SF3B3; RSE1; SAP130; SF3b130; STAF130
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
SF3B3, Antibody; SF3B3 Antibody; KIAA0017 antibody; Pre mRNA splicing factor SF3b 130 kDa subunit antibody; Pre-mRNA-splicing factor SF3b 130 kDa subunit antibody; RSE1 antibody; SAP 130 antibody; SAP130 antibody; SF3b130 antibody; SF3B3 antibody; SF3B3_HUMAN antibody; Spliceosome associated protein 130 antibody; Spliceosome-associated protein 130 antibody; Splicing factor 3b subunit 3 130kD antibody; Splicing factor 3B subunit 3 antibody; STAF130 antibody; anti-SF3B3 antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JG63-53
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-SF3B3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:100
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within the C-terminus of human SF3B3.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of LOVO cells with SF3B3 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining SF3B3 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of SF3B3 on Jurkat and Hela cells lysates using anti-SF3B3 antibody at 1/1, 000 dilution.)

Related Product Information for anti-SF3B3 antibody
SF3B is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B contains the spliceosomal proteins SAP 49, SAP 130 (also known as SF3B3), SAP 145 and SAP 155. SF3B3, SAP 145 and SAP 155 are present in a protein complex in HeLa nuclear extracts and associate with one another. While SF3B3 and SAP 155 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.
Product Categories/Family for anti-SF3B3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
135,577 Da
NCBI Official Full Name
splicing factor 3B subunit 3
NCBI Official Synonym Full Names
splicing factor 3b, subunit 3, 130kDa
NCBI Official Symbol
SF3B3
NCBI Official Synonym Symbols
RSE1; SAP130; SF3b130; STAF130
NCBI Protein Information
splicing factor 3B subunit 3; SAP 130; spliceosome-associated protein 130; pre-mRNA-splicing factor SF3b 130 kDa subunit; pre-mRNA splicing factor SF3b, 130 kDa subunit
UniProt Protein Name
Splicing factor 3B subunit 3
UniProt Gene Name
SF3B3
UniProt Synonym Gene Names
KIAA0017; SAP130; SF3b130; SAP 130
UniProt Entry Name
SF3B3_HUMAN

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Product Notes

The SF3B3 sf3b3 (Catalog #AAA30495) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SF3B3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SF3B3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:100 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the SF3B3 sf3b3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SF3B3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.