FCM (Flow Cytometry)
(Flow Cytometry analyis of SSTR2 in human cell line. Human breast cancer cell line was stained with AAA14651 (filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 secondary antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.)
Mouse anti-Human Somatostatin Receptor Type 2 Monoclonal Antibody | anti-SSTR2 antibody
Somatostatin Receptor Type 2 (SSTr2)
Gene Names
SSTR2; SSTR2
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Synonyms
Somatostatin Receptor Type 2, Antibody; Somatostatin Receptor Type 2 (SSTr2); Anti -Somatostatin Receptor Type 2 (SSTr2); anti-SSTR2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
8B44
Specificity
Recognizes human Somatostatin Receptor Type 2. Stains SSTr2 transfectants but not irrelevant transfectants.
Purity/Purification
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Concentration
~0.5 mg/mL (after reconstitution). (varies by lot)
Applicable Applications for anti-SSTR2 antibody
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC)
Application Notes
Suitable for use in Flow Cytometry, Immunocytochemistry and Immunohistochemistry.
Dilution: Flow Cytometry (Intercellular staining): 2.5ug labels 10e6 cells. Human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin.
Immunocytochemistry: 8-25ug/ml. Immersion fixed MDA-MB-231 human breast cancer cell line.
Immunohistochemistry: 8-25ug/ml. Immersion fixed paraffin-embedded sections of human brain (cingulate cortex)
Optimal dilutions to be determined by researcher.
Dilution: Flow Cytometry (Intercellular staining): 2.5ug labels 10e6 cells. Human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin.
Immunocytochemistry: 8-25ug/ml. Immersion fixed MDA-MB-231 human breast cancer cell line.
Immunohistochemistry: 8-25ug/ml. Immersion fixed paraffin-embedded sections of human brain (cingulate cortex)
Optimal dilutions to be determined by researcher.
Immunogen
Recombinant protein corresponding to Met1-Ile369 from human Somatostatin Receptor Type2, expressed in NSO cells.
Grade
Affinity purified
Preparation and Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
FCM (Flow Cytometry)
(Flow Cytometry analyis of SSTR2 in human cell line. Human breast cancer cell line was stained with AAA14651 (filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 secondary antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.)
FCM (Flow Cytometry)
(Flow Cytometry analyis of SSTR2 in human cell line. Human breast cancer cell line was stained with AAA14651 (filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 secondary antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.)
IHC (Immunohistochemistry)
(Immunohistochemistry analyis of SSTR2 in human cell line. SSTR2 was detected in immersion fixed human breast cancer cell line using AAA14651 (10ug/ml) for 3 hours at RT. Cells were stained with NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (brown) and counterstained with DAPI (blue).)
IHC (Immunohistochemistry)
(Immunohistochemistry analyis of SSTR2 in human cell line. SSTR2 was detected in immersion fixed human breast cancer cell line using AAA14651 (10ug/ml) for 3 hours at RT. Cells were stained with NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (brown) and counterstained with DAPI (blue).)
IHC (Immunohistochemistry)
(Immunohistochemistry analyis of SSTR2 in human brain. SSTR2 was detected in immersion fixed paraffin-embedded sections of human brain (cingulate cortex) using AAA14651 (3ug/ml) overnight at 4°C. Tissue was stained with the Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).)
IHC (Immunohistochemistry)
(Immunohistochemistry analyis of SSTR2 in human brain. SSTR2 was detected in immersion fixed paraffin-embedded sections of human brain (cingulate cortex) using AAA14651 (3ug/ml) overnight at 4°C. Tissue was stained with the Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).)
Related Product Information for anti-SSTR2 antibody
Somatostatin Receptor 2 (SSTr2) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Human SSTr2 shares 84% aa identity with mouse SSTr2 within the extracellular domains. Isoform B (357aa) has an alternate C-terminal cytoplasmic region that is 12aa shorter than that of isoform A (369aa, reported as 93kD). Both are expressed in brain, stomach, intestinal epithelia, pancreatic islets and kidney tubules. Isoform B is also expressed in parotid, thyroid and bronchial glands.
Product Categories/Family for anti-SSTR2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
somatostatin receptor type 2
NCBI Official Synonym Full Names
somatostatin receptor 2
NCBI Official Symbol
SSTR2
NCBI Official Synonym Symbols
SSTR2
NCBI Protein Information
somatostatin receptor type 2; SS2R; SRIF-1
UniProt Protein Name
Somatostatin receptor type 2
UniProt Gene Name
SSTR2
UniProt Entry Name
SSR2_HUMAN
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Product Notes
The SSTR2 sstr2 (Catalog #AAA14651) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Somatostatin Receptor Type 2 (SSTr2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Somatostatin Receptor Type 2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC). Suitable for use in Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Dilution: Flow Cytometry (Intercellular staining): 2.5ug labels 10e6 cells. Human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin. Immunocytochemistry: 8-25ug/ml. Immersion fixed MDA-MB-231 human breast cancer cell line. Immunohistochemistry: 8-25ug/ml. Immersion fixed paraffin-embedded sections of human brain (cingulate cortex) Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the SSTR2 sstr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Somatostatin Receptor Type 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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