<
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with TCF7L2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit TCF7L2 Monoclonal Antibody | anti-TCF7L2 antibody
TCF7L2 Antibody
Gene Names
TCF7L2; TCF4; TCF-4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
TCF7L2, Antibody; TCF7L2 Antibody; HMG box transcription factor 4 antibody; hTCF 4 antibody; hTCF-4 antibody; T cell factor 4 antibody; T cell specific HMG box antibody; T cell specific transcription factor 4 antibody; T-cell factor 4 antibody; T-cell-specific transcription factor 4 antibody; TCF 4 antibody; TCF-4 antibody; TCF4 antibody; TCF7L2 antibody; TCF7L2 protein antibody; TF7L2_HUMAN antibody; Transcription factor 7 like 2 antibody; Transcription factor 7 like 2 T cell specific HMG box antibody; Transcription factor 7-like 2 antibody; anti-TCF7L2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SC06-90
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-TCF7L2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with TCF7L2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with TCF7L2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining TCF7L2 in D3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TCF7L2 in D3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TCF7L2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TCF7L2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TCF7L2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TCF7L2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-TCF7L2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-TCF7L2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TCF7L2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TCF7L2 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of TCF7L2 on Jurkat cells lysates using anti-TCF7L2 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of TCF7L2 on Jurkat cells lysates using anti-TCF7L2 antibody at 1/1, 000 dilution.)
Related Product Information for anti-TCF7L2 antibody
T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to beta-catenin in a cooperative manner. This TCF-4/beta-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes. c-Jun also interacts with TCF-4 and beta-catenin, and the phosphorylation-dependent interaction between c-Jun and TCF4 regulates intestinal tumorigenesis by integrating JNK and APC/beta-catenin. TCF-4 is also implicated in bipolar affective disorder.
Product Categories/Family for anti-TCF7L2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
619
NCBI Official Full Name
Transcription factor 7-like 2
NCBI Official Synonym Full Names
transcription factor 7-like 2 (T-cell specific, HMG-box)
NCBI Official Symbol
TCF7L2
NCBI Official Synonym Symbols
TCF4; TCF-4
NCBI Protein Information
transcription factor 7-like 2; hTCF-4; T-cell factor 4; T-cell factor-4 variant A; T-cell factor-4 variant B; T-cell factor-4 variant C; T-cell factor-4 variant D; T-cell factor-4 variant E; T-cell factor-4 variant F; T-cell factor-4 variant G; T-cell fac
UniProt Protein Name
Transcription factor 7-like 2
UniProt Gene Name
TCF7L2
UniProt Synonym Gene Names
TCF4; T-cell factor 4; TCF-4; hTCF-4
UniProt Entry Name
TF7L2_HUMAN
Similar Products
Product Notes
The TCF7L2 tcf7l2 (Catalog #AAA30140) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TCF7L2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TCF7L2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the TCF7L2 tcf7l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF7L2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
