Mouse anti-Human TIGIT Monoclonal Antibody | anti-TIGIT antibody

TIGIT Antibody [10B1]

Cat. Num. AAA11007

• Gene Names: TIGIT; VSIG9; VSTM3; WUCAM
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Protein A purified
• Synonyms: TIGIT, Antibody; TIGIT Antibody [10B1]; T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; WUCAM; anti-TIGIT antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10B1
• Purity/Purification: Protein A purified
• Form/Format: Liquid; Supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 244

• Applicable Applications for anti-TIGIT antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: IHC starting at 2 ug/mL; IF start at 1 ug/mL; FC/FACS at 1 ug/ml; ICC at 1 ug/mL.; ; Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Immunogen: TIGIT antibody was raised against the extracellular domain of human TIGIT
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.; As with all antibodies care should be taken to avoid repeated freeze thaw cycles.; Antibodies should not be exposed to prolonged high temperatures.

ELISA

(Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA at decreasing concentrations.)

FCM (Flow Cytometry)

(Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)

IHC (Immunohistochemistry)

(Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml.Green: TIGIT Antibody [10B1]Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml.Green: TIGIT Antibody [10B1]Blue: DAPI staining)

ICC (Immunocytochemistry)

(Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)

Related Product Information for anti-TIGIT antibody

The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).

Product Categories/Family for anti-TIGIT antibody

Immunology

References

Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63. Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8(+) T cell effector function. Cancer Cell 2014; 26:923-37.

NCBI and Uniprot Product Information

• NCBI GI #: 256600228
• NCBI GeneID: 201633
• NCBI Accession #: NP_776160
• NCBI GenBank Nucleotide #: NM_173799.3
• UniProt Accession #: Q495A1; Q495A3; Q5JPD8; Q6MZS2; Q8N877
• Molecular Weight: Predicted: 26 kDa; Observed: 47 kDa
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: TIGIT
• NCBI Official Synonym Symbols: VSIG9; VSTM3; WUCAM
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: TIGIT
• UniProt Synonym Gene Names: VSIG9; VSTM3