FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stainedwith Company A (5ug), Company B (5ug), Company C (5ug), Company D (5ug), AAA27031 (5ug) (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 hat 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
Mouse TUBA1A Monoclonal Antibody | anti-TUBA1A antibody
TUBA1A
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01% PBS, pH 7.4
IHC: 1:100-1:300
IF: 1:50-1:200
FC: 1:100-1:300
IP: 1ug-5ug
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stainedwith Company A (5ug), Company B (5ug), Company C (5ug), Company D (5ug), AAA27031 (5ug) (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 hat 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stainedwith Company A (5ug), Company B (5ug), Company C (5ug), Company D (5ug), AAA27031 (5ug) (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 hat 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA27031 (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITCgoat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA27031 (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITCgoat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
IP (Immunoprecipitation)
(Immunoprecipitating TUBA1A in Hela whole cell lysateLane 1: Mouse control IgG (1ug) instead of AAA27031 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Company A (5ug), Company B (5ug), Company C (5ug), Company D (5ug), AAA27031 (5ug) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating TUBA1A in Hela whole cell lysateLane 1: Mouse control IgG (1ug) instead of AAA27031 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Company A (5ug), Company B (5ug), Company C (5ug), Company D (5ug), AAA27031 (5ug) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating TUBA1A in Hela whole cell lysateLane 1: Mouse control IgG (1ug) instead of AAA27031 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA27031 (5ug) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating TUBA1A in Hela whole cell lysateLane 1: Mouse control IgG (1ug) instead of AAA27031 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA27031 (5ug) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells withAAA27031 at 1:75, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488- congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells withAAA27031 at 1:75, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488- congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IHC (Immunohistochemistry)
(IHC image of AAA27031 diluted at 1:150 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA27031 diluted at 1:150 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistchemistry)
(IHC image of AAA27031 diluted at 1:150 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistchemistry)
(IHC image of AAA27031 diluted at 1:150 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysateAll lanes: Company A, Company B, Company C, Company D, AAA27031 antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 320000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysateAll lanes: Company A, Company B, Company C, Company D, AAA27031 antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 320000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysate at 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug, 0.3125ug, 0.15625ugAll lanes: Company A, Company B, Company C, Company D, AAA27031 antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysate at 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug, 0.3125ug, 0.15625ugAll lanes: Company A, Company B, Company C, Company D, AAA27031 antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysateAll lanes: TUBA1A antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysateAll lanes: TUBA1A antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysate at 20ug, 10ug, 5ug, 2.5ug, 1.25ugAll lanes: TUBA1A antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Positive WB detected in: Hela whole cell lysate at 20ug, 10ug, 5ug, 2.5ug, 1.25ugAll lanes: TUBA1A antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Positive WB detected in: NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse brain tissue, Mouse kidney tissueAll lanes: TUBA1A antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Positive WB detected in: NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse brain tissue, Mouse kidney tissueAll lanes: TUBA1A antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Positive WB detected in: Rat brain tissue, Rat stomach tissue, Rat kidney tissueAll lanes: TUBA1A antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Positive WB detected in: Rat brain tissue, Rat stomach tissue, Rat kidney tissueAll lanes: TUBA1A antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Rabbit heart tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissueAll lanes: TUBA1A antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Rabbit heart tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissueAll lanes: TUBA1A antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate,All lanes: TUBA1A antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate,All lanes: TUBA1A antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
NCBI and Uniprot Product Information
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Product Notes
The TUBA1A tuba1a (Catalog #AAA27031) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TUBA1A reacts with Human, Rabbit, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TUBA1A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC), Immunoprecipitation (IP). WB: 1:20000-1:320000 IHC: 1:100-1:300 IF: 1:50-1:200 FC: 1:100-1:300 IP: 1ug-5ug. Researchers should empirically determine the suitability of the TUBA1A tuba1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUBA1A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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