Mouse anti-Human Tubulin alpha-4A Chain Monoclonal Antibody | anti-TUBA4A antibody

Tubulin alpha-4A Chain (TUBA4A, Alpha-tubulin 1, Tubulin alpha-1 Chain, TUBA1, Testis-specific alpha-tubulin, Tubulin H2-alpha) (AP)

Cat. Num. AAA24396

• Gene Names: TUBA4A; ALS22; TUBA1; H2-ALPHA
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Tubulin alpha-4A Chain, Antibody; Tubulin alpha-4A Chain (TUBA4A, Alpha-tubulin 1, Tubulin alpha-1 Chain, TUBA1, Testis-specific alpha-tubulin, Tubulin H2-alpha) (AP); Alpha Tubulin; Tubulin alpha Ubiquitous; H2-Alpha; K-alpha-1; TUBA3; Tubulin alpha 1 Chain; TUBA1; TUBA1A; Tubulin K alpha 1; anti-TUBA4A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2E11
• Specificity: Recognizes human TUBA1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TUBA4A antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-448 from human TUBA1 (AAH09238) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

WB (Western Blot)

(Western Blot analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody. Lane 1: TUBA1 transfected lysate (49.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot)

(TUBA1 monoclonal antibody. Western Blot analysis of TUBA1 expression in HeLa.)

WB (Western Blot)

(Western blot analysis of TUBA1 over-expressed 293 cell line, cotransfected with TUBA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TUBA1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence)

(Immunofluorescence of monoclonal antibody to TUBA1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry)

(Immunoperoxidase of monoclonal antibody to TUBA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])

WB (Western Blot)

(TUBA1 monoclonal antibody. Western Blot analysis of TUBA1 expression in different Cell lines and Human Tissue.)

WB (Western Blot)

(Western Blot detection against Immunogen (75.02kD).)

Product Categories/Family for anti-TUBA4A antibody

Antibodies; Tubulin

NCBI and Uniprot Product Information

• NCBI GI #: 37589861
• NCBI GeneID: 7277
• Molecular Weight: 48,329 Da
• NCBI Official Full Name: Homo sapiens tubulin, alpha 4a, mRNA
• NCBI Official Synonym Full Names: tubulin alpha 4a
• NCBI Official Symbol: TUBA4A
• NCBI Official Synonym Symbols: ALS22; TUBA1; H2-ALPHA
• NCBI Protein Information: tubulin alpha-4A chain