Mouse anti-Human VEGF-A, Biotin-conjugated Monoclonal Antibody | anti-VEGF-A antibody

Mouse Anti-Human VEGF-A

Cat. Num. AAA14942

• Gene Names: VEGFA; VPF; VEGF; MVCD1
• Reactivity: Human
• Applications: ELISA
• Purity: Protein G purified
• Synonyms: VEGF-A, Biotin-conjugated, Antibody; Mouse Anti-Human VEGF-A; Anti-human VEGF-A-Biotin (#339/H2); Vascular endothelial growth factor-A; VPF; anti-VEGF-A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 339/H2
• Purity/Purification: Protein G purified
• Form/Format: Lyophilized
• Sequence Length: 412

• Applicable Applications for anti-VEGF-A antibody: ELISA (EIA)
• Application Notes: ELISA: Use at 1-5 ug/ml
• Immunogen: recombinant human VEGF189
• Conjugation: Biotin
• Preparation: Monoclonals were produced with the help of BALB/c mice using recombinant human VEGF189 derived from E. coli.
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
• Buffer/Stabilizer: PBS; 50X BSA
• Preservative: 0,02% sodium azide
• Warnings: Reagents contain sodium azide. Under acidic conditions sodium azide yields hydrazoic acid, this is extremely toxic. Azide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive condition may develop.
• Preparation and Storage: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. AVOID REPEATED FREEZE AND THAW CYCLES!

Application Data

(VEGF-A Sandwich-ELISA using VEGF189 (Cat#) as standard. Mouse anti-human VEGF-A #3C5 (Cat#) was used as capture antibody, Biotinylated mouse anti-human VEGF-A #339/H2 (Cat# AAA14942) was used for detection.)

Related Product Information for anti-VEGF-A antibody

Human Vascular Endothelial Growth Factor VEGF165, a 23kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.

References

1. Breier et al., Dev 114:521, 1992 2. Fiebig et al., Eur J Biochem 211:19, 1993 3. Flamme et al., Dev Biol 162:699, 1995 4. Kremer at al., Cancer Res 57:3852, 1997

NCBI and Uniprot Product Information

• NCBI GI #: 76781480
• NCBI GeneID: 7422
• NCBI Accession #: NP_001165095
• NCBI GenBank Nucleotide #: NM_001171624
• UniProt Accession #: P15692-2; O60720; O75875; Q074Z4; Q16889; Q5UB46; Q6P0P5; Q96KJ0; Q96L82; Q96NW5; Q9H1W8; B5BU86; P15692
• NCBI Official Full Name: vascular endothelial growth factor A isoform a
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1
• NCBI Protein Information: vascular endothelial growth factor A; vascular permeability factor
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF
• UniProt Entry Name: VEGFA_HUMAN