IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with VEGF Monoclonal Antibody (SPM225).)
Mouse VEGF (Vascular Endothelial Growth Factor) Monoclonal Antibody | anti-VEGFA antibody
VEGF (Vascular Endothelial Growth Factor) Mouse Monoclonal Antibody
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human, Mouse, Rat, Rabbit, Dog. Others not known.
Applications
Immunofluorescence, Immunohistochemistry
Synonyms
VEGF (Vascular Endothelial Growth Factor), Antibody; VEGF (Vascular Endothelial Growth Factor) Mouse Monoclonal Antibody; MVCD1; Vascular Endothelial Growth Factor A (VEGF-A); Vascular Permeability Factor (VPF); VEGFA; anti-VEGFA antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Rabbit, Dog. Others not known.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM225
Specificity
This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
412
Applicable Applications for anti-VEGFA antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (4-8ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed) (4-8ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Cellular Localization
Cytoplasmic, Cell Surface and Extracellular (Secreted)
Immunogen
Human VEGF189 recombinant protein
Hu-Chromosome Location
6p12
Positive Control
Tumor cells in astrocytomas, breast or ovarian carcinomas.
Preparation and Storage
Antibody with azide - store at 2-8°C. Non-hazardous. No MSDS required.
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with VEGF Monoclonal Antibody (SPM225).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with VEGF Monoclonal Antibody (SPM225).)
References
Tischer, E., et al. 1991. The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing. J. Biol. Chem. 266: 11947-11954. Berse, B., et al. 1992. Vascular permeability factor (vascular endothelial growth factor) gene is expressed differentially in normal tissues, macrophages and tumors. Mol. Biol. Cell 3: 211-220. Folkman, J., et al. 1989. Induction of angiogenesis during the transition from hyperplasia to neoplasia. Nature 339: 58-61.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19-22kDa (reducing); 38kDa-44kDa (non-reducing)
NCBI Official Full Name
vascular endothelial growth factor A isoform a
NCBI Official Synonym Full Names
vascular endothelial growth factor A
NCBI Official Symbol
VEGFA
NCBI Official Synonym Symbols
VPF; VEGF; MVCD1
NCBI Protein Information
vascular endothelial growth factor A
UniProt Protein Name
Vascular endothelial growth factor A
UniProt Gene Name
VEGFA
UniProt Synonym Gene Names
VEGF; VEGF-A; VPF
UniProt Entry Name
VEGFA_HUMAN
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Product Notes
The VEGFA vegfa (Catalog #AAA13827) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VEGF (Vascular Endothelial Growth Factor) Mouse Monoclonal Antibody reacts with Human, Mouse, Rat, Rabbit, Dog. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's VEGF (Vascular Endothelial Growth Factor) can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. Immunofluorescence (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (4-8ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the VEGFA vegfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VEGF (Vascular Endothelial Growth Factor), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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