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IF (Immunofluorescence)
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Mouse Vinculin Monoclonal Antibody | anti-VCL antibody

Vinculin Monoclonal Antibody

Gene Names
VCL; MV; MVCL; CMD1W; CMH15
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Protein A/G Purification
Synonyms
Vinculin, Antibody; Vinculin Monoclonal Antibody; Vinculin; Metavinculin (MV); VCL; anti-VCL antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4D4
Purity/Purification
Protein A/G Purification
Form/Format
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-VCL antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:2000-1:4000
IHC: 1:200-1:400
IF: 1:200-1:400
Immunogen
Recombinant human Vinculin protein expressed by E coli
Preparation and Storage
Store at -20 degree C Valid for 12 months. Avoid freeze/thaw cycles. Protected from prolonged exposure to light.

IF (Immunofluorescence)

(Immunofluorescence analysis of C6 cells using Vinculin Monoclonal Antibody at dilution of 1:200.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using Vinculin Monoclonal Antibody at dilution of 1:200.)

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cells using Vinculin Monoclonal Antibody at dilution of 1:200.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Human colon cancer using Vinculin Monoclonal Antibody at dilution of 1:1000.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Human breast cancer using Vinculin Monoclonal Antibody at dilution of 1:1000.)

WB (Western Blot)

(Western blot with Anti Vinculin Monoclonal Antibody at dilution of 1:3000. Lane 1: HeLa cell lysate, Lane 2: 293 cell lysate, Lane 3: Hep G2 cell lysate, Lane 4: NIH/3T3 cell lysate, Lane 5: Mouse heart tissue lysate, Lane 6: Rat heart tissue lysate.)

Related Product Information for anti-VCL antibody
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1, 066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
Product Categories/Family for anti-VCL antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
123,799 Da
NCBI Official Full Name
vinculin isoform VCL
NCBI Official Synonym Full Names
vinculin
NCBI Official Symbol
VCL
NCBI Official Synonym Symbols
MV; MVCL; CMD1W; CMH15
NCBI Protein Information
vinculin; metavinculin
UniProt Protein Name
Vinculin
UniProt Gene Name
VCL
UniProt Synonym Gene Names
MV
UniProt Entry Name
VINC_HUMAN

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Vinculin Antibody

Host: Mouse
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Apps: Western Blot, Immunohistochemistry, Immunofluorescence
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VCL Antibody

Host: Mouse
Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Frog, Fish, Bird
Apps: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
product-image-AAA18464_SDS_PAGE.jpg

VCL Recombinant Protein

Host: E Coli or Yeast or Baculovirus or Mammalian Cell
Reactivity: n/a
Apps: n/a

Product Notes

The VCL vcl (Catalog #AAA22339) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Vinculin Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Vinculin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:2000-1:4000 IHC: 1:200-1:400 IF: 1:200-1:400. Researchers should empirically determine the suitability of the VCL vcl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vinculin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.