Mouse Vinculin Monoclonal Antibody | anti-VCL antibody

Vinculin (Marker of Age-related Macular Degeneration)

Cat. Num. AAA23953

• Gene Names: VCL; MV; MVCL; CMD1W; CMH15; HEL114
• Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Frog, Fish, Bird
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Vinculin, Antibody; Vinculin (Marker of Age-related Macular Degeneration); CMD1W; CMH15; Epididymis luminal protein 114; HEL114; Metavinculin; MV; MVCL; VCL; VINC; anti-VCL antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Frog, Fish, Bird
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: VCL/3617
• Specificity: This antibody recognizes both Vinculin (125kDa) and meta-Vinculin (135kDa). Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1066

• Applicable Applications for anti-VCL antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
• Application Notes: IF: 1-2ug/ml; FC/FACS: 1-2ug/million cells; IHC-P: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes
• Immunogen: Recombinant full-length Human Vinculin protein (VCL)
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 10q22.2
• Positive Control: A431, K562, NIH3T3, U-87, THP-1 cells. Bladder and Testis
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Immunofluorescence Analysis of PFA fixed U87 cells labeling VCL-Monospecific Mouse Monoclonal Antibody (VCL/3617) followed by Goat anti-mouse IgG-CF488 (Green).)

FCM (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed U87 cells using Vinculin Mouse Monoclonal Antibody (VCL/3617) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Mouse Vinculin Monoclonal Antibody (VCL/3617). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis VCL-Monospecific Mouse Monoclonal Antibody(VCL/3617). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry-Formalin)

(Formalin-fixed, paraffin-embedded human testis stained with VCL-Monospecific Mouse Monoclonal Antibody(VCL/3617).)

IHC (Immunohistochemistry-Formalin)

(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with VCL-Monospecific Mouse Monoclonal Antibody (VCL/3617).)

References

Glukhova M.A. et al. Developmental Changes in Expression of Contractile and Cytoskeletal Proteins in Human Aortic Smooth Muscle. J. Biol. Chem., 265: 13042-13046; 1990.

NCBI and Uniprot Product Information

• NCBI GI #: 4507877
• NCBI GeneID: 7414
• NCBI Accession #: NP_003364.1
• NCBI GenBank Nucleotide #: NP_003364.1
• UniProt Accession #: P18206
• Molecular Weight: 125-135kDa
• NCBI Official Full Name: vinculin isoform VCL
• NCBI Official Synonym Full Names: vinculin
• NCBI Official Symbol: VCL
• NCBI Official Synonym Symbols: MV; MVCL; CMD1W; CMH15; HEL114
• NCBI Protein Information: vinculin
• UniProt Protein Name: Vinculin
• UniProt Gene Name: VCL
• UniProt Synonym Gene Names: MV
• UniProt Entry Name: VINC_HUMAN