IP (Immunoprecipitation)
(IP antibody use: 5ug VSV-G Mouse IgG1 per ml Lysate, WB 1:20001, untransfected 293 cell lysate 2, transfected 293 cell lysate with VSV-G-tag fusion protein3, IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4, IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5, IP (transfected 293+anti-VSV-G mAb+ Protein G agarose)6, IP (transfected 293+Protein G)7, Recombinant protein (E.coli))
Mouse VSV-G-Tag Monoclonal Antibody | anti-VSV-G-Tag antibody
VSV-G-Tag Mouse Monoclonal Antibody
Applications
Western Blot, Immunoprecipitation, Immunofluorescence
Synonyms
VSV-G-Tag, Antibody; VSV-G-Tag Mouse Monoclonal Antibody; anti-VSV-G-Tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8D6
Specificity
The VSV-G tag antibody can recognize C-terminal, internal, and N-terminal VSV-G fusion proteins.
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Applicable Applications for anti-VSV-G-Tag antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
WB: 1:1000-1:3000
IP: 1:200
IF: 1:500
IP: 1:200
IF: 1:500
Preparation and Storage
Store at -20°C/1 year
IP (Immunoprecipitation)
(IP antibody use: 5ug VSV-G Mouse IgG1 per ml Lysate, WB 1:20001, untransfected 293 cell lysate 2, transfected 293 cell lysate with VSV-G-tag fusion protein3, IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4, IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5, IP (transfected 293+anti-VSV-G mAb+ Protein G agarose)6, IP (transfected 293+Protein G)7, Recombinant protein (E.coli))
IP (Immunoprecipitation)
(IP antibody use: 5ug VSV-G Mouse IgG1 per ml Lysate, WB 1:20001, untransfected 293 cell lysate 2, transfected 293 cell lysate with VSV-G-tag fusion protein3, IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4, IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5, IP (transfected 293+anti-VSV-G mAb+ Protein G agarose)6, IP (transfected 293+Protein G)7, Recombinant protein (E.coli))
IF (Immunofluorescence)
(IF analysis of 293T cells transfected with a VSV-G-tagged protein, using VSV-G-Tag (8D6) Mouse mAb at a 1:500 dilution (blue DAPI ,red anti-VSV-G).)
IF (Immunofluorescence)
(IF analysis of 293T cells transfected with a VSV-G-tagged protein, using VSV-G-Tag (8D6) Mouse mAb at a 1:500 dilution (blue DAPI ,red anti-VSV-G).)
WB (Western Blot)
(1ug VSV-G fusion protein+ Primary antibody dilution at 1, 1:5,000 (lane 1) and 1:2,000 (lane 2))
WB (Western Blot)
(1ug VSV-G fusion protein+ Primary antibody dilution at 1, 1:5,000 (lane 1) and 1:2,000 (lane 2))
Related Product Information for anti-VSV-G-Tag antibody
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation.
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Product Notes
The VSV-G-Tag (Catalog #AAA27915) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's VSV-G-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). WB: 1:1000-1:3000 IP: 1:200 IF: 1:500. Researchers should empirically determine the suitability of the VSV-G-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VSV-G-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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