Apolipoprotein AI (Apo AI) Native Protein | APOA1 native protein
Human Apolipoprotein AI
Gene Names
APOA1; MGC117399
Applications
SDS-Page
Purity
> 99% pure (SDS-PAGE).
Synonyms
Apolipoprotein AI (Apo AI); N/A; Human Apolipoprotein AI; Human Apolipoprotein AI (Apo AI); APOA1 native protein
Purity/Purification
> 99% pure (SDS-PAGE).
Form/Format
Purified, Liquid ; 3 M Guanidine Hydrochloride, 10mM Tris, 100mM Sodium chloride, 1 mM EDTA, pH 7.4
Concentration
1 mg/mL (OD280nm, E0.1% = 1.13) (varies by lot)
Sequence Length
267
Applicable Applications for APOA1 native protein
SDS-PAGE
Application Notes
Apo AI can be re-natured into PBS, TBS and other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14,000 or less.
This should be performed in a cold room.
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
This should be performed in a cold room.
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
Source
High-density lipoprotein (HDL) from pooled Human plasma
Purification Note
Isolated by ultracentrifugal flotation between densities 1.063–1.21 g/mL, lyophilized then subjected to repeated ethanol extraction. The precipitated protein was dried under nitrogen then dissolved in 6 M Guanidine Hydrochloride -25 mM DTT. Product was then subjected to Sephacryl S200 filtration and eluted.
Preservative
1mM Sodium azide
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Preparation and Storage
Short-term store at 2-8°C. Long term store at -20°C.Avoid multiple freeze/thaw cycles.
Dialyzed Apo AI in PBS or TBS with 1mM EDTA and 0.1% Sodium Azide should be stored for up to 2 weeks at 2 to 8°C.
Storage of Apo A1 in PBS or TBS at -20°C may lead to precipitation when thawed.
Dialyzed Apo AI in PBS or TBS with 1mM EDTA and 0.1% Sodium Azide should be stored for up to 2 weeks at 2 to 8°C.
Storage of Apo A1 in PBS or TBS at -20°C may lead to precipitation when thawed.
Related Product Information for APOA1 native protein
Human Apoliprotein A1
Human Apoliprotein A1 (Apo A1)
Human Apoliprotein A1 (Apo A1)
Product Categories/Family for APOA1 native protein
References
• Chen, W., et al., (2001), "Preferential ATP-binding Cassette Transporter A1-mediated Cholesterol Efflux from Late Endosomes/Lysosomes", The Journal of Biological Chemistry, 276(47): 43564-43569
• Feng, B., et al., (2002), "ABCA1-m
• Feng, B., et al., (2002), "ABCA1-m
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
apolipoprotein AI
NCBI Official Synonym Full Names
apolipoprotein A-I
NCBI Official Symbol
APOA1
NCBI Official Synonym Symbols
MGC117399
NCBI Protein Information
apolipoprotein A-I; apo-AI; apoA-I; OTTHUMP00000043268; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348
UniProt Protein Name
Apolipoprotein A-I
UniProt Gene Name
APOA1
UniProt Entry Name
APOA1_HUMAN
Similar Products
Product Notes
The APOA1 apoa1 (Catalog #AAA13427) is a Native Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Apolipoprotein AI (Apo AI) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Apo AI can be re-natured into PBS, TBS and other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14,000 or less. This should be performed in a cold room. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Researchers should empirically determine the suitability of the APOA1 apoa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apolipoprotein AI (Apo AI), Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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