Application Data
NP protein of Zarie Ebolavirus (isolate H.sapiens-wt/GIN/2014/Kissidougou-C15) Recombinant Protein | NP recombinant protein
NP protein (aa 396-738) of Zarie Ebolavirus (isolate H.sapiens-wt/GIN/2014/Kissidougou-C15)
Applications
ELISA, Western Blot
Purity
>= 95% (by SDS-PAGE)
Synonyms
NP protein of Zarie Ebolavirus (isolate H.sapiens-wt/GIN/2014/Kissidougou-C15); N/A; NP protein (aa 396-738) of Zarie Ebolavirus (isolate H.sapiens-wt/GIN/2014/Kissidougou-C15); NP Protein of Zarie Ebolavirus; NP Protein of Zarie Ebolavirus (isolate H.sapiens-wt/GIN/2014/Kissidougou-C15); NP recombinant protein
Host
293 cell culture
Purity/Purification
>= 95% (by SDS-PAGE)
Concentration
1 ug/ul in PBS (varies by lot)
Sequence
TLRKE RLAKLTEAIT AASLPKTSGH YDDDDDIPFP GPINDDDNPG HQDDDPTDSQ DTTIPDVVVD PDDGGYGEYQ SYSENGMSAP DDLVLFDLDE DDEDTKPVPN RSTKGGQQKN SQKGQHTEGR QTQSTPTQNV TGPRRTIHHA SAPLTDNDRR NEPSGSTSPR MLTPINEEAD PLDDADDETS SLPPLESDDE EQDRDGTSNR TPTVAPPAPV YRDHSEKKEL PQDEQQDQDH IQEARNQDSD NTQPEHSFEE MYRHILRSQG PFDAVLYYHM MKDEPVVFST SDGKEYTYPD SLEEEYPPWL TEKEAMNDEN RFVTLDGQQF YWPVMNHRNK FMAILQHH
Applicable Applications for NP recombinant protein
ELISA, WB (Western Blot)
Viral Protein
C-terminal 6x His tagged nucleoprotein (NP) (amino acid 396-738) of Zarie Ebolavirus (isolate H.sapiens wt/GIN/2014/Kissidougou-C15) (Genebank No. AHX24646.1)
Endotoxin level
<0.01 EU per 1 ug of the protein by LAL test
Preparation and Storage
Store at -20°C. Stable for 6-months from the date of shipment when kept at 4°C. Non-hazardous. No MSDS required.
Application Data
Application Data
Related Product Information for NP recombinant protein
Description:
Viral protein purified from 293 cell culture
Introduction: The Ebola virus (EBOV) is a mononegavirus which contains a 19 kb single-stand RNA encoding seven proteins: nucleoprotein (NP), viral protein (VP) 35, VP40, glycoprotein (GP), VP30, VP24, and RNA polymerase (L). VP30, VP35, NP, and L are the 4 virion structural proteins. VP40, GP, and VP24 are the 3 membrane-associated proteins.
NP plays an important role in the replication of the viral genome and is essential for formation of the nucleocapsid. The produced NP protein (~45 kDa) is derived from the sequence of a recent Zarie Ebolavirus (ZEBOV) isolate from 2014 outbreak in western Africa.
Viral protein purified from 293 cell culture
Introduction: The Ebola virus (EBOV) is a mononegavirus which contains a 19 kb single-stand RNA encoding seven proteins: nucleoprotein (NP), viral protein (VP) 35, VP40, glycoprotein (GP), VP30, VP24, and RNA polymerase (L). VP30, VP35, NP, and L are the 4 virion structural proteins. VP40, GP, and VP24 are the 3 membrane-associated proteins.
NP plays an important role in the replication of the viral genome and is essential for formation of the nucleocapsid. The produced NP protein (~45 kDa) is derived from the sequence of a recent Zarie Ebolavirus (ZEBOV) isolate from 2014 outbreak in western Africa.
Product Categories/Family for NP recombinant protein
References
Baize, S, et al. Emergence of Zaire Ebola virus disease in Guinea. N. Engl. J. Med., 371: 1418-1425, 2014.
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Product Notes
The NP (Catalog #AAA62210) is a Recombinant Protein produced from 293 cell culture and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NP protein of Zarie Ebolavirus (isolate H.sapiens-wt/GIN/2014/Kissidougou-C15) can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TLRKE RLAKLTEAIT AASLPKTSGH YDDDDDIPFP GPINDDDNPG HQDDDPTDSQ DTTIPDVVVD PDDGGYGEYQ SYSENGMSAP DDLVLFDLDE DDEDTKPVPN RSTKGGQQKN SQKGQHTEGR QTQSTPTQNV TGPRRTIHHA SAPLTDNDRR NEPSGSTSPR MLTPINEEAD PLDDADDETS SLPPLESDDE EQDRDGTSNR TPTVAPPAPV YRDHSEKKEL PQDEQQDQDH IQEARNQDSD NTQPEHSFEE MYRHILRSQG PFDAVLYYHM MKDEPVVFST SDGKEYTYPD SLEEEYPPWL TEKEAMNDEN RFVTLDGQQF YWPVMNHRNK FMAILQHH. It is sometimes possible for the material contained within the vial of "NP protein of Zarie Ebolavirus (isolate H.sapiens-wt/GIN/2014/Kissidougou-C15), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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