Application Data
opa gene recombinant protein
opa gene recombinant
Applications
Western Blot
Purity
0.98
Synonyms
opa gene; N/A; opa gene recombinant; RECOMBINANT ANTIGEN opa from Neisseria meningitidis serogroup C; opacity-associated adhesin proteins; opa gene recombinant protein
Host
E Coli
Purity/Purification
0.98
Form/Format
Dry powder (lyophilized)
Concentration
3.30 mg/ml (varies by lot)
Sequence Length
256
Applicable Applications for opa gene recombinant protein
WB (Western Blot)
Recombinant Antigen
Neisseria meningitidis serogroup C opacity protein and related surface antigen (Virji et al., 1993).
Organism
Neisseria meningitidis serotype C
Tag
HIS
Molecular Weight Information
Determined by SDS-PAGE, the protein band runs between molecular markers of 25,000 and 35,000 Da, while relative molecular mass calculated from amino acid sequence is 32,272.5 Da.
Observation Notes
Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
Buffer
20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether, 250 mM Imidazol, 8 M urea
Batch Composition
Recombinant antigen with a his-tag in its N-terminus
Storage Buffer
20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether, 250 mM Imidazol, 8 M urea
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Application Data
Application Data
Application Data
Application Data
Related Product Information for opa gene recombinant protein
the Neisseria meningitidis serogroup C gene for the opacity protein has been expressed as a recombinant antigen fused to a his-tag in its N-terminus. It contains aa 30 to 251 of the complete 256 aa-protein.
Product Categories/Family for opa gene recombinant protein
References
Virji, M., K. Makepeace, D. J. Ferguson, M. Achtman, and E. R. Moxon. Meningococcal Opa and Opc proteins: their role in colonization and invasion of humanepithelial and endothelial cells. 1993, Mol. Microbiol. 10:499- 510.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
opacity outermembrane protein
Similar Products
Product Notes
The opa gene (Catalog #AAA72853) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's opa gene can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the opa gene for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "opa gene, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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