Application Data
PI4K2B recombinant protein
PI4K2B, Active
Gene Names
PI4K2B; PIK42B; PI4KIIB
Applications
Kinase Assay
Synonyms
PI4K2B; N/A; PI4K2B, Active; PI4K2B recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
Full length
Sequence Length
3601
Applicable Applications for PI4K2B recombinant protein
Kinase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for PI4K2B recombinant protein
Description: Full-length recombinant human PI4K2Beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PI4K2B is a member of the phosphatidylinositol 4-kinase family (PI4K) that phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate which are immediate precursor of several important signaling and scaffolding molecules. PI4K2B is primarily a cytosolic PI4K that is recruited to membranes where it stimulates phosphatidylinositol 4,5-bisphosphate syntheses (1). PI4K2B uses phosphatidylinositol as their primary substrate and has no activity on phosphatidylinositol monophosphates. PI4K2B is a restricted minor histocompatibility antigen in patients that had been successfully treated with donor lymphocyte infusions for relapsed chronic myeloid leukemia after allogeneic stem cell transplantation (2).
Scientific Background: PI4K2B is a member of the phosphatidylinositol 4-kinase family (PI4K) that phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate which are immediate precursor of several important signaling and scaffolding molecules. PI4K2B is primarily a cytosolic PI4K that is recruited to membranes where it stimulates phosphatidylinositol 4,5-bisphosphate syntheses (1). PI4K2B uses phosphatidylinositol as their primary substrate and has no activity on phosphatidylinositol monophosphates. PI4K2B is a restricted minor histocompatibility antigen in patients that had been successfully treated with donor lymphocyte infusions for relapsed chronic myeloid leukemia after allogeneic stem cell transplantation (2).
Product Categories/Family for PI4K2B recombinant protein
References
1. Balla, A. et.al: Characterization of type II phospha-tidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments. J. Biol. Chem. 277: 20041-20050, 2002. 2. Griffioen, M. et.al: Identification of phosphatidylinositol 4-kinase type II beta as HLA class II-restricted target in graft versus leukemia reactivity. Proc. Nat. Acad. Sci. 105: 3837-3842, 2008.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~84 kDa
NCBI Official Full Name
Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphatidylinositol 4-kinase type 2 beta
NCBI Official Symbol
PI4K2B
NCBI Official Synonym Symbols
PIK42B; PI4KIIB
NCBI Protein Information
phosphatidylinositol 4-kinase type 2-beta
UniProt Protein Name
Phosphatidylinositol 4-kinase type 2-beta
UniProt Gene Name
PI4K2B
UniProt Synonym Gene Names
PI4KII-BETA
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Product Notes
The PI4K2B pi4k2b (Catalog #AAA73049) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full length. AAA Biotech's PI4K2B can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the PI4K2B pi4k2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI4K2B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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