Application Data
PIK3C2A recombinant protein
PIK3C2A, Active
Gene Names
PIK3C2A; CPK; PI3-K-C2A; PI3K-C2alpha; PI3K-C2-alpha; PI3-K-C2(ALPHA)
Applications
Kinase Assay
Synonyms
PIK3C2A; N/A; PIK3C2A, Active; PIK3C2A recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
299-end
Sequence Length
8452
Applicable Applications for PIK3C2A recombinant protein
Kinase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for PIK3C2A recombinant protein
Description: Recombinant human PIK3C2A (299-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PIK3C2A is belongs to the phosphoinositide 3-kinase (PI3K) family which play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. PIK3C2A can phosphorylate phosphatidylinositol and phosphatidylinositol-4-phosphate in vitro (1). PIK3C2A contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases that act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions (2).
Scientific Background: PIK3C2A is belongs to the phosphoinositide 3-kinase (PI3K) family which play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. PIK3C2A can phosphorylate phosphatidylinositol and phosphatidylinositol-4-phosphate in vitro (1). PIK3C2A contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases that act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions (2).
Product Categories/Family for PIK3C2A recombinant protein
References
1. Domin, J. et.al: Cloning of a human phosphoinositide 3-kinase with a C2 domain that displays reduced sensitivity to the inhibitor wortmannin. Biochem. J. 326: 139-147, 1997. 2. Molz, L. et.al: Cpk is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain. J. Biol. Chem. 271: 13892-13899, 1996.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
155 kDa
NCBI Official Full Name
Homo sapiens phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha (PIK3C2A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
NCBI Official Symbol
PIK3C2A
NCBI Official Synonym Symbols
CPK; PI3-K-C2A; PI3K-C2alpha; PI3K-C2-alpha; PI3-K-C2(ALPHA)
NCBI Protein Information
phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
UniProt Protein Name
Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
UniProt Gene Name
PIK3C2A
UniProt Synonym Gene Names
PI3K-C2-alpha; PtdIns-3-kinase C2 subunit alpha
Similar Products
Product Notes
The PIK3C2A pik3c2a (Catalog #AAA73050) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 299-end. AAA Biotech's PIK3C2A can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the PIK3C2A pik3c2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3C2A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.