NAD(P) transhydrogenase subunit alpha Recombinant Protein | pntA recombinant protein

Recombinant Escherichia coli NAD(P) transhydrogenase subunit alpha

Cat. Num. AAA115309

• Gene Names: pntA; ECK1598; JW1595
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: NAD(P) transhydrogenase subunit alpha; N/A; Recombinant Escherichia coli NAD(P) transhydrogenase subunit alpha; Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha; pntA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-401
• Sequence: MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTT
• Sequence Length: 510

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for pntA recombinant protein

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.

References

Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli.Clarke D.M., Loo T.W., Gillam S., Bragg P.D.Eur. J. Biochem. 158:647-653(1986) A mutation at Gly314 of the beta subunit of the Escherichia coli pyridine nucleotide transhydrogenase abolishes activity and affects the NADP(H) -induced conformational change.Ahmad S., Glavas N.A., Bragg P.D.Eur. J. Biochem. 207:733-739(1992) A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.DNA Res. 3:363-377(1996) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Topological analysis of the pyridine nucleotide transhydrogenase of Escherichia coli using proteolytic enzymes.Tong R.C., Glavas N.A., Bragg P.D.Biochim. Biophys. Acta 1080:19-28(1991) Global topology analysis of the Escherichia coli inner membrane proteome.Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.Science 308:1321-1323(2005) X-ray structure of domain I of the proton-pumping membrane protein transhydrogenase from Escherichia coli.Johansson T., Oswald C., Pedersen A., Toernroeth S., Okvist M., Karlsson B.G., Rydstroem J., Krengel U.J. Mol. Biol. 352:299-312(2005) Structure determination of a transient complex by NMR using paramagnetic distance restraints - the complex of the soluble domains of Escherichia coli transhydrogenase.Johansson T., Pedersen A., Leckner J., Karlsson B.G.Submitted (MAY-2005) to the PDB data bank

NCBI and Uniprot Product Information

• NCBI GI #: 16129561
• NCBI GeneID: 946628
• NCBI Accession #: NP_416120.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P07001; P76888
• Molecular Weight: 44.8 kDa
• NCBI Official Full Name: pyridine nucleotide transhydrogenase, alpha subunit
• NCBI Official Symbol: pntA
• NCBI Official Synonym Symbols: ECK1598; JW1595
• NCBI Protein Information: pyridine nucleotide transhydrogenase, alpha subunit
• UniProt Protein Name: NAD(P) transhydrogenase subunit alpha
• UniProt Gene Name: pntA
• UniProt Entry Name: PNTA_ECOLI