Rabbit anti-Human ADIPOR2 Polyclonal Antibody | anti-ADIPOR2 antibody

ADIPOR2, ID (ADIPOR2, PAQR2, Adiponectin receptor protein 2, Progestin and adipoQ receptor family member II)

Cat. Num. AAA14774

• Gene Names: ADIPOR2; PAQR2; ACDCR2
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Immunofluorescence
• Purity: This antibody is purified through a protein Acolumn, followed by peptide affinity purification.
• Synonyms: ADIPOR2, Antibody; ADIPOR2, ID (ADIPOR2, PAQR2, Adiponectin receptor protein 2, Progestin and adipoQ receptor family member II); Anti -ADIPOR2, ID (ADIPOR2, PAQR2, Adiponectin receptor protein 2, Progestin and adipoQ receptor family member II); anti-ADIPOR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ADIPOR2 antibody is generated from rabbits immunized with KLH conjugted synthetic peptide between 45-72 amino acids from the Central region of human ADIPOR2.
• Purity/Purification: This antibody is purified through a protein Acolumn, followed by peptide affinity purification.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
• Concentration: 0.39 mg/mL (varies by lot)

• Applicable Applications for anti-ADIPOR2 antibody: Suitable for use in ELISA (EIA), Western Blot (WB), Flow Cytometry (FC), Immunofluorescence (IF)
• Application Notes: WB~~1:1000; FC~~1:25; IF~~1:10~50
• Immunogen: KLH conjugated synthetic peptide mapping to a fragment of residues within amino acids 45-72 in the central region of human ADIPOR2, UniProt Accession #Q86V24.
• Function: Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (PubMed:12802337, PubMed:25855295). Required for normal body at and glucose homeostasis. ADIPOQ-binding activates a signaling cascade that leads to increased PPARA activity, and ultimately to increased fatty acid oxidation and glucose uptake. Has intermediate affinity for globular and full-length adiponectin. Required for normal revascularization after chronic ischemia caused by severing of blood vessels (By similarity).
• Cellular Location: Cell membrane; Multi-pass membrane protein Note=Localized to the cell membrane and intracellular organelles
• Tissue Location: Ubiquitous (PubMed:16044242). Highly expressed in skeletal muscle, liver and placenta (PubMed:12802337). Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung (PubMed:12802337)
• Antigen Region: 45-72
• Precautions: ADIPO2 Antibody (center) is for research use only
• Preparation and Storage: Maintain refrigerated at 2°C to 8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM (Flow Cytometry)

(Flow cytometric analysis of Hela cells (right histogram) using AAA14774 compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of Hela cell using AAA14774 followed by Alexa Fluor489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot)

(Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human skeletal muscle tissue lysateLysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human liver tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-ADIPOR2 Antibody (Center) at 1:2000 dilution Lane 1: U-251 MG whole cell lysateLane 2: U-2OS whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Western Blot analysis in Hela cell line lysates (35ug/lane) uusing of AAA14774. ADIPOR2 (arrow) was detected using AAA14774.)

Related Product Information for anti-ADIPOR2 antibody

The adiponectin receptors, ADIPOR1 (MIM 607945) and ADIPOR2, serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin.

Product Categories/Family for anti-ADIPOR2 antibody

Antibodies; Abs to Obesity Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 38261973
• NCBI GeneID: 79602
• NCBI Accession #: NP_078827.2
• NCBI GenBank Nucleotide #: NM_024551.2
• UniProt Accession #: Q86V24; Q53YY5; Q9H737
• NCBI Official Full Name: adiponectin receptor protein 2
• NCBI Official Synonym Full Names: adiponectin receptor 2
• NCBI Official Symbol: ADIPOR2
• NCBI Official Synonym Symbols: PAQR2; ACDCR2
• NCBI Protein Information: adiponectin receptor protein 2; progestin and adipoQ receptor family member II
• UniProt Protein Name: Adiponectin receptor protein 2
• UniProt Gene Name: ADIPOR2
• UniProt Synonym Gene Names: PAQR2
• UniProt Entry Name: ADR2_HUMAN