IF (Immunofluorescence)
(AAA13648 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
Goat AKAP9/AKAP450/CG-NAP Polyclonal Antibody | anti-AKAP9 antibody
Goat anti-AKAP9/AKAP450/CG-NAP Antibody
Gene Names
AKAP9; LQT11; PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; PPP1R45; HYPERION; MU-RMS-40.16A
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
AKAP9/AKAP450/CG-NAP, Antibody; Goat anti-AKAP9/AKAP450/CG-NAP Antibody; AKAP9, CG-NAP, PRKA9, YOTIAO, AKAP350, HYPERION, KIAA0803, A kinase (PRKA) anchor protein (yotiao) 9, AKAP120-like protein, kinase N-associated protein, A-kinase anchoring protein 450, A-kinase anchor protein, 350kDa, protein kinase A anchoring protein 9, centrosome- and golgi-localized protein, AKAP450, MU-RMS-40.16A, A-kinase anchor protein 9, AKAP9-BRAF fusion protein; anti-AKAP9 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Applicable Applications for anti-AKAP9 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: This product has been successfully used by a customer, showing a band at approx 450kDa in lysates of cell line HepG2 (calculated MW of 453kDa according to NP_005742.4). Recommended concentration: 1-3ug/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10ug/ml.
Western blot: This product has been successfully used by a customer, showing a band at approx 450kDa in lysates of cell line HepG2 (calculated MW of 453kDa according to NP_005742.4). Recommended concentration: 1-3ug/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10ug/ml.
Immunogen
Peptide with sequence C-SGSTTQFHAGMR, from the C Terminus of the protein sequence according to NP_005742.4; NP_671714.1.
Note
This antibody is expected to recognise isoforms 2 and 3 (NP_005742.4; NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
IF (Immunofluorescence)
(AAA13648 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
IF (Immunofluorescence)
(AAA13648 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
A-kinase anchor protein 9 isoform 2
NCBI Official Synonym Full Names
A-kinase anchoring protein 9
NCBI Official Symbol
AKAP9
NCBI Official Synonym Symbols
LQT11; PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; PPP1R45; HYPERION; MU-RMS-40.16A
NCBI Protein Information
A-kinase anchor protein 9
UniProt Protein Name
A-kinase anchor protein 9
UniProt Gene Name
AKAP9
UniProt Synonym Gene Names
AKAP350; AKAP450; KIAA0803; PRKA9
UniProt Entry Name
AKAP9_HUMAN
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Product Notes
The AKAP9 akap9 (Catalog #AAA13648) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-AKAP9/AKAP450/CG-NAP Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AKAP9/AKAP450/CG-NAP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: This product has been successfully used by a customer, showing a band at approx 450kDa in lysates of cell line HepG2 (calculated MW of 453kDa according to NP_005742.4). Recommended concentration: 1-3ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10ug/ml. Researchers should empirically determine the suitability of the AKAP9 akap9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKAP9/AKAP450/CG-NAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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