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IS (Immunostaining)
(Immunostaining of 25?m thick cryosections of PFA-perfused Mouse Amygdala, antigen retrieval withcitrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.)
Goat Androgen Receptor Polyclonal Antibody | anti-AR antibody
Goat anti-Androgen Receptor Antibody
Gene Names
AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Tested: Human, MouseExpected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Androgen Receptor, Antibody; Goat anti-Androgen Receptor Antibody; AR; androgen receptor; dihydrotestosterone receptor; KD; AIS; TFM; DHTR; SBMA; NR3C4; SMAX1; HUMARA; HYSP1; RP11-383C12.1; androgen receptor antibody; dihydrotestosterone receptor antibody; AR antibody; KD antibody; AIS antibody; TFM antibody; DHTR antibody; SBMA antibody; NR3C4 antibody; SMAX1 antibody; HUMARA antibody; HYSP1 antibody; RP11-383C12.1 antibody; Androgen Receptor; anti-AR antibody
Host
Goat
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence
EVQLGLGRVYPRPPSC
Sequence Length
920
Applicable Applications for anti-AR antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Negative Control
Application Notes
Peptide ELISA:
antibody detection limit dilution 1:64000.
Western blot:
Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5ug/ml. Primary incubation 1 hour at room temperature.
Negative Control:
Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.
Immunohistochemistry:
In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3ug/ml. Cryosections of Mouse Hypothalamus and Amygdala. Recommended dilution range: 1:20,000
Immunofluorescence:
Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10ug/ml.
Chromatin Immunoprecipitation:
A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using AAA13656 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.
antibody detection limit dilution 1:64000.
Western blot:
Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5ug/ml. Primary incubation 1 hour at room temperature.
Negative Control:
Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.
Immunohistochemistry:
In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3ug/ml. Cryosections of Mouse Hypothalamus and Amygdala. Recommended dilution range: 1:20,000
Immunofluorescence:
Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10ug/ml.
Chromatin Immunoprecipitation:
A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using AAA13656 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.
Immunogen
Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.
Note
This antibody is expected to recognize isoform 1 (NP_000035.2) only.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
IS (Immunostaining)
(Immunostaining of 25?m thick cryosections of PFA-perfused Mouse Amygdala, antigen retrieval withcitrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.)
IS (Immunostaining)
(Immunostaining of 25?m thick cryosections of PFA-perfused Mouse Amygdala, antigen retrieval withcitrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.)
IS (Immunostaining)
(Immunostaining of 25µm thick cryosections of PFA-perfused Mouse Hypothalamus, antigen retrieval with citrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.)
IS (Immunostaining)
(Immunostaining of 25µm thick cryosections of PFA-perfused Mouse Hypothalamus, antigen retrieval with citrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.)
WB (Western Blot)
((0.5µg/ml) staining of Human Cerebellum (A) and (0.1ug/ml) negative control Pancreas (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
((0.5µg/ml) staining of Human Cerebellum (A) and (0.1ug/ml) negative control Pancreas (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.)
IF (Immunofluorescence)
(AAA13656 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(AAA13656 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(AAA13656 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(AAA13656 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
ChIP (Chromatin Immunoprecipitation)
(ChIP of 2ug AAA13656 with 1ug DHT-treated HEC50 chromatin using the Chromatrap spin column sonication kit (Protein G) measuring FKBP5 enrichment.)
ChIP (Chromatin Immunoprecipitation)
(ChIP of 2ug AAA13656 with 1ug DHT-treated HEC50 chromatin using the Chromatrap spin column sonication kit (Protein G) measuring FKBP5 enrichment.)
IHC (Immunohistochemistry)
(AAA13656 (2ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
(AAA13656 (2ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
androgen receptor isoform 1
NCBI Official Synonym Full Names
androgen receptor
NCBI Official Symbol
AR
NCBI Official Synonym Symbols
KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
NCBI Protein Information
androgen receptor
UniProt Protein Name
Androgen receptor
UniProt Gene Name
AR
UniProt Synonym Gene Names
DHTR; NR3C4
UniProt Entry Name
ANDR_HUMAN
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Product Notes
The AR ar (Catalog #AAA13656) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Androgen Receptor Antibody reacts with Tested: Human, Mouse Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Androgen Receptor can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Negative Control. Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch. Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3ug/ml. Cryosections of Mouse Hypothalamus and Amygdala. Recommended dilution range: 1:20,000 Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10ug/ml. Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using AAA13656 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells. Researchers should empirically determine the suitability of the AR ar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EVQLGLGRVY PRPPSC. It is sometimes possible for the material contained within the vial of "Androgen Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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