Rabbit ANKRD33B Polyclonal Antibody | anti-ANKRD33B antibody
ANKRD33B Polyclonal Antibody
IHC (Immunohistochemistry-Paraffin)
(Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (ANKRD33B) Polyclonal Antibody, Unconjugated (bs-9397R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistchemistry-Paraffin)
(Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (ANKRD33B) Polyclonal Antibody, Unconjugated (bs-9397R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (ANKRD33B) Polyclonal Antibody, Unconjugated (bs-9397R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
WB (Western Blot)
(Sample: Cerebral cortex (Rat) Lysate at 40 ug Primary: Anti- ANKRD33B (bs-9397R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD)
WB (Western Blot)
(Dilution: Sample: Cerebellum (Mouse) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Primary: Anti- ANKRD33B (bs-9397R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD)
WB (Western Blot)
(Sample: Cerebellum (Mouse) Lysate at 40 ug Cerebellum (Rat) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Primary: Anti- ANKRD33B (bs-9397R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD)
WB (Western Blot)
(Sample: Cerebral cortex (Mouse) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- ANKRD33B (bs-9397R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 52 kD)