AOC3 / VAP-1 Polyclonal Antibody | anti-AOC3 antibody

Goat Polyclonal to Human AOC3 / VAP-1

Cat. Num. AAA12319

• Gene Names: AOC3; HPAO; SSAO; VAP1; VAP-1
• Reactivity: Human, Hamster; Predicted Reactivity: Monkey, Mouse, Rat, Rabbit
• Applications: Immunohistochemistry, Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: AOC3 / VAP-1, Antibody; Goat Polyclonal to Human AOC3 / VAP-1; Anti-AOC3 / VAP-1 Antibody (Internal) IHC-plus; AOC3; Copper amine oxidase; HPAO; Membrane primary amine oxidase; SSAO; VAP-1; VAP1; Vascular adhesion protein 1; Human AOC3; anti-AOC3 antibody

• Host: AOC3/VAP-1 antibody was produced in Goat
• Reactivity: Human, Hamster; Predicted Reactivity: Monkey, Mouse, Rat, Rabbit
• Clonality: Polyclonal
• Specificity: Human AOC3 / VAP-1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: TBS, pH 7.3, 0.02% sodium azide, 0.5% BSA
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Positions: aa712-724
• Sequence Length: 724

• Applicable Applications for anti-AOC3 antibody: Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-Paraffin), Peptide Enzyme-Linked Immunosorbent Assay
• Application Notes: Immunohistochemistry-Paraffin (IHC-Paraffin) (3.75 ug/ml); Peptide Enzyme-Linked Immunosorbent Assay (1:64000); ; Optimal dilutions to be determined by the researcher).
• Target: AOC3/VAP-1
• Immunogen Species: AOC3/VAP-1 antibody was raised against Human
• Antigen Type: Synthetic peptide
• Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 3.75 ug/ml.
• Immunogen: AOC3 / VAP-1 antibody was raised against synthetic peptide C-DEDPSFYSADSIY from an internal region (near the C-terminus) of human AOC3 / VAP-1 (NP_003725.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Marmoset, Hamster (100%); Gibbon, Monkey, Mouse, Rat, Rabbit (92%); Elephant, Bovine (85%).
• Antigen Modification: Internal
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C. Avoid freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 4502119
• NCBI GeneID: 8639
• NCBI Accession #: NP_003725.1
• NCBI GenBank Nucleotide #: NM_003734.3
• UniProt Accession #: Q16853; Q45F94; B2RCI5; K7ESB3; L0L8N9
• NCBI Official Full Name: membrane primary amine oxidase isoform 1
• NCBI Official Synonym Full Names: amine oxidase, copper containing 3
• NCBI Official Symbol: AOC3
• NCBI Official Synonym Symbols: HPAO; SSAO; VAP1; VAP-1
• NCBI Protein Information: membrane primary amine oxidase; copper amine oxidase; placenta copper monamine oxidase; semicarbazide-sensitive amine oxidase; amine oxidase, copper containing 3 (vascular adhesion protein 1)
• UniProt Protein Name: Membrane primary amine oxidase
• UniProt Gene Name: AOC3
• UniProt Synonym Gene Names: VAP1; SSAO; VAP-1
• UniProt Entry Name: AOC3_HUMAN