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IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using AAA28677 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Rabbit ATG7 Polyclonal Antibody | anti-ATG7 antibody
ATG7 Antibody (C-term)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human, MousePredicted reactivity: Chicken, Rat
Applications
Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ATG7, Antibody; ATG7 Antibody (C-term); Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein; ATG7; APG7L; anti-ATG7 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted reactivity: Chicken, Rat
Predicted reactivity: Chicken, Rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human ATG7.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
540-569
Sequence Length
623
Applicable Applications for anti-ATG7 antibody
Western Blot (WB), ELISA (EIA), ImmunohistochemistryParaffin-Leica (IHC), Immunofluorescence (IF)
Application Notes
WB~~1:500
IF~~1:25
IHC-P-Leica~~1:1000
IF~~1:25
IHC-P-Leica~~1:1000
Cellular location
Cytoplasm. Preautophagosomal structure.Note=Localizes also to discrete punctaealong the ciliary axoneme and to the base ofthe ciliary axoneme
Tissue location
Widely expressed, especially in kidney, liver,lymph nodes and bone marrow.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using AAA28677 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using AAA28677 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of APG7L Pab in 293 cell line lysate (35ug/lane). APG7L (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of APG7L Pab in 293 cell line lysate (35ug/lane). APG7L (arrow) was detected using the purified Pab.)
IF (Immunofluorescence)
(Immunofluorescent analysis of U251 cells, using ATG7 Antibody (C-term). U251 cells(right) were treated with Chloroquine (50 uM,16h). AAA28677 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of U251 cells, using ATG7 Antibody (C-term). U251 cells(right) were treated with Chloroquine (50 uM,16h). AAA28677 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)
Application Data
(All lanes : Anti-ATG7 Antibody (C-term) at1:1000 dilution Lane 1: HepG2 whole cell lysateLane 2: NIH/3T3 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 78 kDa Blocking/Dilution buffer: 5%)
Application Data
(All lanes : Anti-ATG7 Antibody (C-term) at1:1000 dilution Lane 1: HepG2 whole cell lysateLane 2: NIH/3T3 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 78 kDa Blocking/Dilution buffer: 5%)
Application Data
(All lanes : Anti-ATG7 Antibody (C-term) at1:500 dilution Lane 1: Caki-1 whole cell lysateLane 2: Hela whole cell lysate Lane 3: HepG2whole cell lysate Lysates/proteins at 40 µg perlane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 78 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.)
Application Data
(All lanes : Anti-ATG7 Antibody (C-term) at1:500 dilution Lane 1: Caki-1 whole cell lysateLane 2: Hela whole cell lysate Lane 3: HepG2whole cell lysate Lysates/proteins at 40 µg perlane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 78 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.)
Related Product Information for anti-ATG7 antibody
E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity)
Product Categories/Family for anti-ATG7 antibody
References
References for protein:
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4.Levine B. Cell. 120(2):159-62. (2005)
5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6.Tanida I., et al. Biochem. Biophys. Res. Commun. 292:256-262(2002)
7.Tanida I., et al. J. Biol. Chem. 277:13739-13744(2002)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4.Levine B. Cell. 120(2):159-62. (2005)
5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6.Tanida I., et al. Biochem. Biophys. Res. Commun. 292:256-262(2002)
7.Tanida I., et al. J. Biol. Chem. 277:13739-13744(2002)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-like modifier-activating enzyme ATG7 isoform b
NCBI Official Synonym Full Names
autophagy related 7
NCBI Official Symbol
ATG7
NCBI Official Synonym Symbols
GSA7; APG7L; APG7-LIKE
NCBI Protein Information
ubiquitin-like modifier-activating enzyme ATG7
UniProt Protein Name
Ubiquitin-like modifier-activating enzyme ATG7
UniProt Gene Name
ATG7
UniProt Synonym Gene Names
APG7L; APG7-like; hAGP7
UniProt Entry Name
ATG7_HUMAN
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Product Notes
The ATG7 atg7 (Catalog #AAA28677) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 540-569. The ATG7 Antibody (C-term) reacts with Human, Mouse Predicted reactivity: Chicken, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATG7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), ImmunohistochemistryParaffin-Leica (IHC), Immunofluorescence (IF). WB~~1:500 IF~~1:25 IHC-P-Leica~~1:1000. Researchers should empirically determine the suitability of the ATG7 atg7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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