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IF (Immunofluorescence)
(IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Rabbit Band 3 Polyclonal Antibody | anti-Band 3 antibody
Anti-Band 3 Antibody
Gene Names
SLC4A1; DI; FR; SW; WD; WR; AE1; CHC; SAO; WD1; BND3; EPB3; SPH4; CD233; EMPB3; RTA1A
Reactivity
Human, Mouse , Rat.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Synonyms
Band 3, Antibody; Anti-Band 3 Antibody; Band 3 anion transport protein; AE 1; AE1; Anion exchange protein 1; Anion exchanger 1; B3AT_HUMAN; BND3; CD233; DI; Diego blood group; EMPB3; EPB3; Erythrocyte membrane protein band 3; Erythroid anion exchange protein; FR; Froese blood group; RTA1A; SLC4A1; Solute carrier family 4 anion exchanger member 1; Solute carrier family 4 member 1; SW; Swann blood group; Waldner blood group; WD; WD1; WR; Wright blood group; solute carrier family 4 (anion exchanger), member 1 (Diego blood group); anti-Band 3 antibody
Host
Rabbit
Reactivity
Human, Mouse , Rat.
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Sequence Length
911
Applicable Applications for anti-Band 3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin
Application Notes
Immunofluorescence:
Concentration: 2ug/ml
Tested Species: Human, Rat
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Human, Rat
Immunohistochemistry (Paraffin-embedded Section)
Concentration: 0.5-1ug/ml
Tested Species: Human, Mouse, Rat
Antigen Retrieval: By Heat
Immunohistochemistry (Frozen Section)
Concentration: 0.5-1ug/ml
Tested Species: Human
Tested Species: In-house tested species with positive results
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Concentration: 2ug/ml
Tested Species: Human, Rat
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Human, Rat
Immunohistochemistry (Paraffin-embedded Section)
Concentration: 0.5-1ug/ml
Tested Species: Human, Mouse, Rat
Antigen Retrieval: By Heat
Immunohistochemistry (Frozen Section)
Concentration: 0.5-1ug/ml
Tested Species: Human
Tested Species: In-house tested species with positive results
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Immunogen
E Coli-derived human Band 3 (Position: E28-N365). Human Band 3 shares 75.7% and 74.5% amino acid (aa) sequence identity with mouse and rat Band 3, respectively.
Gene Name
Solute carrier family 4 (annion exchanger), member 1 (Diego blood group)
Recommended Protein Name
Band 3 anion transport protein.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection systems
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (please inquire) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (please inquire) for IHC(P) and IHC(F).
Preparation and Storage
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C. Avoid repeated freeze-thaw cycles
IF (Immunofluorescence)
(IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IHC (Immunohistochemistry)
(Anti- Band 3 Picoband antibody, AAA11648,IHC(F)IHC(F): Human Placenta Tissue)
IHC (Immunohistochemistry)
(Anti- Band 3 Picoband antibody, AAA11648,IHC(F)IHC(F): Human Placenta Tissue)
IHC (Immunohistochemistry)
(Anti- Band 3 Picoband antibody,IHC(P)IHC(P): Mouse Kidney Tissue)
IHC (Immunohistochemistry)
(Anti- Band 3 Picoband antibody,IHC(P)IHC(P): Mouse Kidney Tissue)
IHC (Immunohistochemistry)
(Anti- Band 3 Picoband antibody,IHC(P)IHC(P): Human Placenta Tissue)
IHC (Immunohistochemistry)
(Anti- Band 3 Picoband antibody,IHC(P)IHC(P): Human Placenta Tissue)
Application Data
(Anti- Band 3 Picoband antibody, AAA11648, IHC(P)IHC(P): Rat Kidney Tissue)
Application Data
(Anti- Band 3 Picoband antibody, AAA11648, IHC(P)IHC(P): Rat Kidney Tissue)
WB (Western Blot)
(Anti- Band 3 Picoband antibody, Western blottingAll lanes: Anti Band 3 at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugLane 4: Rat Spleen Tissue Lysate at 50ugLane 5: Human Placenta Tissue Lysate at 50ugPredicted bind size: 102KDObserved bind size: 102KD)
WB (Western Blot)
(Anti- Band 3 Picoband antibody, Western blottingAll lanes: Anti Band 3 at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugLane 4: Rat Spleen Tissue Lysate at 50ugLane 5: Human Placenta Tissue Lysate at 50ugPredicted bind size: 102KDObserved bind size: 102KD)
Related Product Information for anti-Band 3 antibody
Background: Band 3 is also known as SLC4A1. The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system.
References
1. Jarolim P, Palek J, Amato D, Hassan K, Sapak P, Nurse GT et al. (1991). "Deletion in erythrocyte band 3 gene in malaria-resistant Southeast Asian ovalocytosis".Proc. Natl. Acad. Sci. U.S.A. 88 (24): 11022-6. 2. Schlüter K, Drenckhahn D (August 1986)."Co-clustering of denatured hemoglobin with band 3: its role in binding of autoantibodies against band 3 to abnormal and aged erythrocytes". Proc. Natl. Acad. Sci. U.S.A. 83 (16): 6137-41.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
band 3 anion transport protein
NCBI Official Synonym Full Names
solute carrier family 4 member 1 (Diego blood group)
NCBI Official Symbol
SLC4A1
NCBI Official Synonym Symbols
DI; FR; SW; WD; WR; AE1; CHC; SAO; WD1; BND3; EPB3; SPH4; CD233; EMPB3; RTA1A
NCBI Protein Information
band 3 anion transport protein
UniProt Protein Name
Band 3 anion transport protein
UniProt Gene Name
SLC4A1
UniProt Synonym Gene Names
AE1; DI; EPB3; AE 1; Anion exchanger 1
UniProt Entry Name
B3AT_HUMAN
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Product Notes
The Band 3 slc4a1 (Catalog #AAA11648) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Band 3 Antibody reacts with Human, Mouse, Rat. and may cross-react with other species as described in the data sheet. AAA Biotech's Band 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin. Immunofluorescence: Concentration: 2ug/ml Tested Species: Human, Rat Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Human, Rat Immunohistochemistry (Paraffin-embedded Section) Concentration: 0.5-1ug/ml Tested Species: Human, Mouse, Rat Antigen Retrieval: By Heat Immunohistochemistry (Frozen Section) Concentration: 0.5-1ug/ml Tested Species: Human Tested Species: In-house tested species with positive results By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end user. Researchers should empirically determine the suitability of the Band 3 slc4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Band 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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