IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using BRCA2 antibody (AAA10700) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Rabbit anti-Human , Rat BRCA2 Polyclonal Antibody | anti-BRCA2 antibody
BRCA2
Gene Names
BRCA2; FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
Reactivity
Human , Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Synonyms
BRCA2, Antibody; BRCA2; anti-BRCA2 antibody
Host
Rabbit
Reactivity
Human , Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Sequence Length
3418
Applicable Applications for anti-BRCA2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IF: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BRCA2 (NP_000050.2)
Species Reactivity/Cross Reactivity
Human, Rat
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using BRCA2 antibody (AAA10700) at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using BRCA2 antibody (AAA10700) at dilution of 1:100. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of 293T cells, using BRCA2 antibody (AAA10700) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of extracts of 293T cells, using BRCA2 antibody (AAA10700) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-BRCA2 antibody
Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.
Product Categories/Family for anti-BRCA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 384 kDa
Calculated MW: 384 kDa
Calculated MW: 384 kDa
NCBI Official Full Name
Breast cancer type 2 susceptibility protein
NCBI Official Synonym Full Names
breast cancer 2, early onset
NCBI Official Symbol
BRCA2
NCBI Official Synonym Symbols
FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
NCBI Protein Information
breast cancer type 2 susceptibility protein; Fanconi anemia group D1 protein; breast cancer 2 tumor suppressor; BRCA1/BRCA2-containing complex, subunit 2; breast and ovarian cancer susceptibility gene, early onset
UniProt Protein Name
Breast cancer type 2 susceptibility protein
UniProt Gene Name
BRCA2
UniProt Synonym Gene Names
FACD; FANCD1
UniProt Entry Name
BRCA2_HUMAN
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Product Notes
The BRCA2 brca2 (Catalog #AAA10700) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRCA2 reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BRCA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500 - 1:2000 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the BRCA2 brca2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRCA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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