Application Data
Rabbit c-Src (Tyr-215) Polyclonal Antibody | anti-SRC antibody
c-Src (Tyr-215), phospho-specific [Conserved site]
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot
Synonyms
c-Src (Tyr-215), Antibody; c-Src (Tyr-215), phospho-specific [Conserved site]; c-Src (Tyr-215); phospho-specific; Src; anti-SRC antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-c-Src (Tyr-215) peptide (without carrier). The antibody detects a 60 kDa* protein on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and human A431 cells that have been treated with pervanadate.
Applicable Applications for anti-SRC antibody
ELISA, Western Blot
Application Notes
ELISA: 1:2000
Western Blot: 1:1000
Western Blot: 1:1000
Immunogen
c-Src (Tyr-215) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 215 of human c-Src. This sequence has homology to the conserved site in other Src family members, such as hck, fyn, csk, and yes.
Buffer
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Preparation and Storage
Store at -20°C.Stable for 1 year.
Application Data
Application Data
Related Product Information for anti-SRC antibody
c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a
variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c- Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 419 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor
activation. Both Tyr-215 and Tyr-419 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src
kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.
References
• Stover, D.R. et al. (1996) J Biol Chem 271(21):12481.
• Vadlamudi, R.K. et al. (2003) FEBS Letters 543:76.
• Vadlamudi, R.K. et al. (2003) FEBS Letters 543:76.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
c-src
NCBI Official Synonym Full Names
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
NCBI Official Symbol
SRC
NCBI Official Synonym Symbols
ASV; SRC1; c-SRC; p60-Src
NCBI Protein Information
proto-oncogene tyrosine-protein kinase Src; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; proto-oncogene c-Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; protooncogene SRC, Rous sarcoma
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase Src
UniProt Gene Name
SRC
UniProt Synonym Gene Names
SRC1
UniProt Entry Name
SRC_HUMAN
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Product Notes
The SRC src (Catalog #AAA14100) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The c-Src (Tyr-215), phospho-specific [Conserved site] reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's c-Src (Tyr-215) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. ELISA: 1:2000 Western Blot: 1:1000. Researchers should empirically determine the suitability of the SRC src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Src (Tyr-215), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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