KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: CASP3 knockout Jurkat cell lysate; Predicted MW: 31kDa Observed MW: 31kDa Primary Ab: 5ug/ml Rabbit Anti-Human CASP3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Rabbit Caspase 3 Polyclonal Antibody | anti-CASP3 antibody
Polyclonal Antibody to Caspase 3 (CASP3)
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Rat, Pig
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Caspase 3, Antibody; Polyclonal Antibody to Caspase 3 (CASP3); anti-CASP3 antibody
Host
Rabbit
Reactivity
Human, Rat, Pig
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CASP3. It has beenselected for its ability to recognize CASP3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
277
Applicable Applications for anti-CASP3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
CASP3 (Ser29~Asp175)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant CASP3 (Ser29~Asp175) expressed in E Coli.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: CASP3 knockout Jurkat cell lysate; Predicted MW: 31kDa Observed MW: 31kDa Primary Ab: 5ug/ml Rabbit Anti-Human CASP3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: CASP3 knockout Jurkat cell lysate; Predicted MW: 31kDa Observed MW: 31kDa Primary Ab: 5ug/ml Rabbit Anti-Human CASP3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Human Jurkat Cells; Lane3: Human Hela Cells.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Human Jurkat Cells; Lane3: Human Hela Cells.)
Product Categories/Family for anti-CASP3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,608 Da
NCBI Official Full Name
caspase-3 isoform a preproprotein
NCBI Official Synonym Full Names
caspase 3
NCBI Official Symbol
CASP3
NCBI Official Synonym Symbols
CPP32; SCA-1; CPP32B
NCBI Protein Information
caspase-3
UniProt Protein Name
Caspase-3
UniProt Gene Name
CASP3
UniProt Synonym Gene Names
CPP32; CASP-3; CPP-32; SCA-1
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Product Notes
The CASP3 casp3 (Catalog #AAA20845) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Caspase 3 (CASP3) reacts with Human, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CASP3 casp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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