Rabbit Catenin-gamma Polyclonal Antibody | anti-CTNNG antibody

Catenin-gamma Antibody

Cat. Num. AAA31082

• Gene Names: JUP; DP3; PDGB; PKGB; CTNNG; DPIII
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: Catenin-gamma, Antibody; Catenin-gamma Antibody; ARVD 12; ARVD12; Catenin (cadherin associated protein), gamma 80kDa; catenin (cadherin-associated protein) gamma (80kD); Catenin gamma; Desmoplakin 3; Desmoplakin III; Desmoplakin-3; Desmoplakin3; DesmoplakinIII; DP 3; DP III; DP3; DPIII; gamma catenin; Junction plakoglobin; JUP; OTTHUMP00000164732; OTTHUMP00000164735; OTTHUMP00000164738; PDGB; PKGB; PLAK_HUMAN; PLAKOGLOBIN; anti-CTNNG antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Catenin-gamma antibody detects endogenous levels of total Catenin-gamma
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 745

• Applicable Applications for anti-CTNNG antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide derived from human Catenin-gamma
• Subcellular Location: Cell Junction > Adherens Junction. Cell Junction > Desmosome. Cytoplasm > Cytoskeleton. Membrane. Cytoplasmic in a soluble and membrane-associated form.
• Predicted Cross Reactivity: Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
• Similarity: Pig (92%), Bovine (92%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (80%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of Catenin-gamma expression in mouse tissue extract)

IHC (Immunohistochemistry)

(Catenin-gamma Antibody for IHC in human colon tissue.)

IHC (Immunohistochemistry)

(Catenin-gamma Antibody for IHC in human colon tissue.)

IHC (Immunohistchemistry)

(AAA31082 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry)

(AAA31082 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot)

(Western blot analysis of extracts from rat brain, mouse spleen, using Catenin-gamma Antibody.)

IF (Immunofluorescence)

(AAA31082 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry)

(AAA31082 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry)

(AAA31082 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry)

(AAA31082 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry)

(AAA31082 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-CTNNG antibody

Function: Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).
Subunit Structure: Homodimer. Component of an E-cadherin/catenin adhesion complex composed of at least E-cadherin/CDH1 and gamma-catenin/JUP, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Interacts with MUC1. Interacts with CAV1 (By similarity). Interacts with PTPRJ. Interacts with DSG1. Interacts with DSC1 and DSC2. Interacts with PKP2.
Post-translational Modifications: May be phosphorylated by FER.
Similarity: The entire ARM repeats region mediates binding to CDH1/E-cadherin. The N-terminus and first three ARM repeats are sufficient for binding to DSG1. The N-terminus and first ARM repeat are sufficient for association with CTNNA1. DSC1 association requires both ends of the ARM repeat region. Belongs to the beta-catenin family.

NCBI and Uniprot Product Information

• NCBI GI #: 4504811
• NCBI GeneID: 3728
• NCBI Accession #: NP_002221.1
• NCBI GenBank Nucleotide #: NM_002230.3
• UniProt Accession #: P14923; Q15093; Q15151; Q7L3S5; Q86W21; Q9BWC4; Q9HCX9
• Molecular Weight: Observed: 80 kDa; Predicted: 82 kDa
• NCBI Official Full Name: junction plakoglobin
• NCBI Official Synonym Full Names: junction plakoglobin
• NCBI Official Symbol: JUP
• NCBI Official Synonym Symbols: DP3; PDGB; PKGB; CTNNG; DPIII
• NCBI Protein Information: junction plakoglobin
• UniProt Protein Name: Junction plakoglobin
• UniProt Gene Name: JUP
• UniProt Synonym Gene Names: CTNNG; DP3