Rabbit anti-Human CB2 Polyclonal Antibody | anti-CNR2 antibody

CB2 Antibody (C-term)

Cat. Num. AAA28672

• Gene Names: CNR2; CB2; CX5; CB-2
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Flow Cytometry, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: CB2, Antibody; CB2 Antibody (C-term); Cannabinoid receptor 2; CB-2; CB2; hCB2; CX5; CNR2; CB2A; CB2B; anti-CNR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This CB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-356 amino acids from the C-terminal region of human CB2.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 329-356
• Sequence Length: 360

• Applicable Applications for anti-CNR2 antibody: ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC), Western Blot (WB)
• Application Notes: IHC-P~~1:10~50; FC~~1:25; WB~~1:1000
• Cellular Location: Cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Perikaryon Note=Localizes to apical dendrite of pyramidal neurons.
• Tissue Location: Preferentially expressed in cells of the immune system with higher expression in B-cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root ganglion sensory neurons (at protein level). Two isoforms are produced by alternative promoter usage and differ only in the 5'UTR: isoform CB2A is observed predominantly in testis with some expression in brain, while isoform CB2B is predominant in spleen and leukocytes
• Function: Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM (Flow Cytometry)

(CB2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry)

(CB2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CB2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(CB2 Antibody (C-term) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the CB2 antibody detected the CB2 protein (arrow).)

FCM (Flow Cytometry)

(Flow cytometric analysis of Jurkat cells using Park7 (DJ-1) Antibody(green) compared to an isotype control of rabbit IgG(blue). AAA28672 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded H. brain section using CB2 Antibody(C-term). AAA28672 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded R. brain section using CB2 Antibody(C-term). AAA28672 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Related Product Information for anti-CNR2 antibody

The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors.

Product Categories/Family for anti-CNR2 antibody

Crown Antibodies; Neuroscience; Loading Control

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Larrinaga, G., et al. Histol. Histopathol. 25(9):1133-1138(2010)
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Taylor, A.H., et al. Histochem. Cell Biol. 133(5):557-565(2010)
De Jesus, M.L., et al. Neurochem. Int. 56 (6-7), 829-833 (2010) :

NCBI and Uniprot Product Information

• NCBI GI #: 4502929
• NCBI GeneID: 1269
• NCBI Accession #: NP_001832.1
• NCBI GenBank Nucleotide #: NM_001841.2
• UniProt Accession #: P34972; Q4VBK8; Q5JRH7; Q6B0G7; Q6NSY0; C6ES44
• NCBI Official Full Name: cannabinoid receptor 2
• NCBI Official Synonym Full Names: cannabinoid receptor 2 (macrophage)
• NCBI Official Symbol: CNR2
• NCBI Official Synonym Symbols: CB2; CX5; CB-2
• NCBI Protein Information: cannabinoid receptor 2
• UniProt Protein Name: Cannabinoid receptor 2
• UniProt Gene Name: CNR2
• UniProt Synonym Gene Names: CB2A; CB2B; CB-2; CB2; hCB2
• UniProt Entry Name: CNR2_HUMAN