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FCM (Flow Cytometry)
(CCR7 Antibody (N-term) (AAA28701) flow cytometric analysis of 293 cells (right histogram) compared to a negative controlcell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse CCR7 Polyclonal Antibody | anti-CCR7 antibody
CCR7 Antibody (N-term)
Gene Names
CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CCR7, Antibody; CCR7 Antibody (N-term); C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR-7; BLR2; CDw197; Epstein-Barr virus-induced G-protein coupled receptor 1; EBI1; EBV-induced G-protein coupled receptor 1; MIP-3 beta receptor; CD197; CCR7; CMKBR7; EVI1; anti-CCR7 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CCR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-37 amino acids from the N-terminal region of human CCR7.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
11-37
Sequence Length
378
Applicable Applications for anti-CCR7 antibody
WB, IHC-P, IF , FC, E
Application Notes
WB~~1:500-1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
Function
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
Cellular Location
Cell membrane; Multi-pass membrane protein.
Tissue Location
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM (Flow Cytometry)
(CCR7 Antibody (N-term) (AAA28701) flow cytometric analysis of 293 cells (right histogram) compared to a negative controlcell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM (Flow Cytometry)
(CCR7 Antibody (N-term) (AAA28701) flow cytometric analysis of 293 cells (right histogram) compared to a negative controlcell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CCR7Antibody (N-term)(AAA28701) withMDA-MB231 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG(green).DAPI was used to stain the cell nuclear(blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CCR7Antibody (N-term)(AAA28701) withMDA-MB231 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG(green).DAPI was used to stain the cell nuclear(blue).)
IHC (Immunohistchemistry)
(CCR7 Antibody (N-term) (AAA28701) IHC analysis in formalin fixed and paraffinembedded human tonsil followed byperoxidase conjugation of the secondaryantibody and DAB staining. This datademonstrates the use of the CCR7 Antibody(N-term) for immunohistochemistry. Clinicalrelevance has not been evaluated.)
IHC (Immunohistchemistry)
(CCR7 Antibody (N-term) (AAA28701) IHC analysis in formalin fixed and paraffinembedded human tonsil followed byperoxidase conjugation of the secondaryantibody and DAB staining. This datademonstrates the use of the CCR7 Antibody(N-term) for immunohistochemistry. Clinicalrelevance has not been evaluated.)
WB (Western Blot)
(CCR7 Antibody (N-term) (AAA28701)western blot analysis in 293(lane1),Ramos(lane 2),MDA-MB231(lane 3) cell lineand mouse spleen tissue(lane 4) lysates(35ug/lane).This demonstrates the CCR7antibody detected the CCR7 protein (arrow).)
WB (Western Blot)
(CCR7 Antibody (N-term) (AAA28701)western blot analysis in 293(lane1),Ramos(lane 2),MDA-MB231(lane 3) cell lineand mouse spleen tissue(lane 4) lysates(35ug/lane).This demonstrates the CCR7antibody detected the CCR7 protein (arrow).)
WB (Western Blot)
(Anti-CCR7 Antibody (Nterm) at 1:500 dilution+ MOLT-4 whole cell lysate Lysates/proteins at20 µg per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CCR7 Antibody (Nterm) at 1:500 dilution+ MOLT-4 whole cell lysate Lysates/proteins at20 µg per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-CCR7 Antibody (N-term) at 1:500-1:1000 dilution Lane 1: MDA-MB-231whole cell lysate Lane 2: MOLT-4 whole celllysate Lysates/proteins at 20 µg per lane.Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 43 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-CCR7 Antibody (N-term) at 1:500-1:1000 dilution Lane 1: MDA-MB-231whole cell lysate Lane 2: MOLT-4 whole celllysate Lysates/proteins at 20 µg per lane.Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 43 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.)
Related Product Information for anti-CCR7 antibody
CCR7 is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor.
Product Categories/Family for anti-CCR7 antibody
References
Sun, J., et al. Cell. Mol. Immunol. 7(1):77-82(2010) Wu, W.L., et al. Eur. J. Immunol. 39(12):3413-3422(2009) Marcenaro, E., et al. Blood 114(19):4108-4116(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
C-C chemokine receptor type 7
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 7
NCBI Official Symbol
CCR7
NCBI Official Synonym Symbols
BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
NCBI Protein Information
C-C chemokine receptor type 7
UniProt Protein Name
C-C chemokine receptor type 7
UniProt Gene Name
CCR7
UniProt Synonym Gene Names
CMKBR7; EBI1; EVI1; C-C CKR-7; CC-CKR-7; CCR-7; EBI1; EBV-induced G-protein coupled receptor 1
UniProt Entry Name
CCR7_HUMAN
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Product Notes
The CCR7 ccr7 (Catalog #AAA28701) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 11-37. The CCR7 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CCR7 can be used in a range of immunoassay formats including, but not limited to, WB, IHC-P, IF, FC, E. WB~~1:500-1:1000IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50. Researchers should empirically determine the suitability of the CCR7 ccr7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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