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IHC (Immunohistochemistry)
(IHC of rat spleen using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:500/DAB detection.)
Goat CD45 Polyclonal Antibody | anti-CD45 antibody
CD45 Polyclonal Antibody
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Reacts against Human, Rat, Mouse, Canine, and Monkey proteins.
Applications
Western Blot
Synonyms
CD45, Antibody; CD45 Polyclonal Antibody; Anti-CD45; B220; CD45 antigen; CD45R; GP180; L-CA; LCA; leukocyte common antigen; LY5; protein tyrosine phosphatase; PTPRC; receptor type c polypeptide; receptor-type tyrosine-protein phosphatase C; RP11-553K8.4; T200; T200 glycoprotein; T200 leukocyte common antigen antibody; anti-CD45 antibody
Host
Goat
Reactivity
Reacts against Human, Rat, Mouse, Canine, and Monkey proteins.
Clonality
Polyclonal
Specificity
Using Jurkat cell lysate detects a band 180-220 kDa by Western blot.
Form/Format
Polyclonal antibody supplied as a 500 ul (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Applicable Applications for anti-CD45 antibody
Western Blot (WB)
Application Notes
WB 1:500-1:2,000
IHC (F) 1:250-1:1,000
IHC (P) 1:250-1:1,000
IHC (F) 1:250-1:1,000
IHC (P) 1:250-1:1,000
Immunogen
Purified recombinant peptide derived from within residues 1,260 aa to the C-terminus of human CD45 produced in E. coli.
Preparation and Storage
Store at -20°C for long-term storage.
Store at 2-8°C for up to one month.
Avoid freeze/thaw cycles.
Store at 2-8°C for up to one month.
Avoid freeze/thaw cycles.
IHC (Immunohistochemistry)
(IHC of rat spleen using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:500/DAB detection.)
IHC (Immunohistochemistry)
(IHC of rat spleen using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:500/DAB detection.)
IHC (Immunohistchemistry)
(IHC of mouse liver using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:500/DAB detection.)
IHC (Immunohistchemistry)
(IHC of mouse liver using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:500/DAB detection.)
IHC (Immunohistochemistry)
(IHC of human lymph node using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:500/DAB detection.)
IHC (Immunohistochemistry)
(IHC of human lymph node using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:500/DAB detection.)
IHC (Immunohistochemistry)
(IHC of mouse eye using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of mouse eye using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of human appendix using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of human appendix using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:750/DAB detection.)
Application Data
Application Data
Reactivity Chart
Reactivity Chart
Related Product Information for anti-CD45 antibody
Goat polyclonal antibody to CD45. It is a 180-220 kDa type I transmembrane glycoprotein and a member of the protein tyrosine phosphatase (PTP) family. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It is expressed in all hematopoietic cells, but highest on lymphocytes. PTPs are known to be signalling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. It has intrinsic tyrosine phosphatase activity and it is essential for T and B cell antigen receptor-mediated activation. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signalling. CD45 also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signalling. The cross-linking of CD45 induces apoptosis in lymphocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CD45
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
PTPRC
NCBI Official Synonym Symbols
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
PTPRC
UniProt Synonym Gene Names
CD45; L-CA
UniProt Entry Name
PTPRC_HUMAN
Similar Products
Product Notes
The CD45 ptprc (Catalog #AAA13860) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The CD45 Polyclonal Antibody reacts with Reacts against Human, Rat, Mouse, Canine, and Monkey proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's CD45 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB 1:500-1:2,000 IHC (F) 1:250-1:1,000 IHC (P) 1:250-1:1,000. Researchers should empirically determine the suitability of the CD45 ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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