Rabbit CHRM4 / M4 Polyclonal Antibody | anti-CHRM4 antibody

Rabbit Polyclonal (IgG) to Human CHRM4 / M4

Cat. Num. AAA12369

• Gene Names: CHRM4; HM4; M4R
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Immunoaffinity Purified
• Synonyms: CHRM4 / M4, Antibody; Rabbit Polyclonal (IgG) to Human CHRM4 / M4; CHRM4 Antibody, M4 muscarinic receptor Antibody, M4R Antibody, Muscarinic m4 receptor Antibody, HM4 Antibody, Muscarinic receptor m4 Antibody; anti-CHRM4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CHRM4 Antibody detects endogenous levels of total CHRM4 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 479

• Applicable Applications for anti-CHRM4 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Peptide Enzyme-Linked Immunosorbent Assay
• Application Notes: IF: 1:100-1:500; IHC-P: 1:50; Optimal dilution to be determined by the researcher.
• Immunogen: CHRM4 / M4 antibody was raised against synthetic peptide from human CHRM4 (aa236-285).
• Immunogen Species: CHRM4 / M4 antibody was raised against human
• Epitope: aa236-285
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C for up to one year.

IF (Immunofluorescence)

(Immunofluorescence of LOVO cells, using CHRM4 Antibody. The picture on the right is treated with the synthesized peptide.)

IHC (Immunohistochemistry)

(Anti-CHRM4 / M4 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Product Categories/Family for anti-CHRM4 antibody

Family: GPCR; Subfamily: Acetylcholine

NCBI and Uniprot Product Information

• NCBI GI #: 52426748
• NCBI GeneID: 1132
• NCBI Accession #: NP_000732.2
• NCBI GenBank Nucleotide #: NM_000741.2
• UniProt Accession #: P08173; Q0VD60; Q4VBK7; B2RPP4
• Molecular Weight: Western Blot: Predicted MW 53kDa
• NCBI Official Full Name: muscarinic acetylcholine receptor M4
• NCBI Official Synonym Full Names: cholinergic receptor, muscarinic 4
• NCBI Official Symbol: CHRM4
• NCBI Official Synonym Symbols: HM4; M4R
• NCBI Protein Information: muscarinic acetylcholine receptor M4; acetylcholine receptor, muscarinic 4
• UniProt Protein Name: Muscarinic acetylcholine receptor M4
• UniProt Gene Name: CHRM4
• UniProt Entry Name: ACM4_HUMAN