WB (Western Blot)
(Figure 9 WB Validation of CXCR4 in metastatic melanoma (Scala et al., 2006)CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.)
Rabbit anti-Human, Mouse CXCR4 Polyclonal Antibody | anti-CXCR4 antibody
CXCR4 Antibody
WB (Western Blot)
(Figure 9 WB Validation of CXCR4 in metastatic melanoma (Scala et al., 2006)CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.)
WB (Western Blot)
(Figure 9 WB Validation of CXCR4 in metastatic melanoma (Scala et al., 2006)CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.)
WB (Western Blot)
(Figure 8 Overexpression Validation of CXCR4 (Kozak et al., 2002)U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.)
WB (Western Blot)
(Figure 8 Overexpression Validation of CXCR4 (Kozak et al., 2002)U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.)
FCM (Flow Cytometry)
(Figure 7 KO Validation of CXCR4 by Flow Cytometry (Ödemis, et al., 2010)Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITC-labeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4?/? astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).)
FCM (Flow Cytometry)
(Figure 7 KO Validation of CXCR4 by Flow Cytometry (Ödemis, et al., 2010)Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITC-labeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4?/? astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).)
IHC (Immunohistchemistry)
(Figure 6 Immunohistochemistry Validation of CXCR4Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody (1012) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistchemistry)
(Figure 6 Immunohistochemistry Validation of CXCR4Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody (1012) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of CXCR4Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with 1012 at 4 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cell line.)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of CXCR4Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with 1012 at 4 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cell line.)
WB (Western Blot)
(Figure 4 Animal Species ReactivityLoading: Lysates/proteins at 20 μg per lane.Antibodies: 1009 (2 μg/mL) or 1012 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 4 Animal Species ReactivityLoading: Lysates/proteins at 20 μg per lane.Antibodies: 1009 (2 μg/mL) or 1012 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 3 Validation with CXCR4 siRNA KnockdownHeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 μg of HeLa whole cell lysates per lane. Antibodies: 1012 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 3 Validation with CXCR4 siRNA KnockdownHeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 μg of HeLa whole cell lysates per lane. Antibodies: 1012 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1012 (1 μg/mL), and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.)
WB (Western Blot)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1012 (1 μg/mL), and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.)
WB (Western Blot)
(Figure 1 Western Blot Validation of CXCR4 in HeLa CellsLoading: 15 μg of lysates per lane.Antibodies: 1012 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 1 Western Blot Validation of CXCR4 in HeLa CellsLoading: 15 μg of lysates per lane.Antibodies: 1012 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
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Product Notes
The CXCR4 cxcr4 (Catalog #AAA10962) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CXCR4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CXCR4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). CXCR4 antibody can be used for Western blot 0.5 - 1 mug/mL. For immunofluorescence start at 4 mug/mL. Researchers should empirically determine the suitability of the CXCR4 cxcr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCR4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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