Rabbit cytomegalovirus (HCMV) Glycoprotein B/gB Polyclonal Antibody | anti-gB antibody
Human cytomegalovirus (HCMV) Glycoprotein B/gB Antibody, Rabbit PAb, Antigen Affinity Purified
Reactivity
CMV
Applications
ELISA
Purity
Protein A & Antigen Affinity
Synonyms
cytomegalovirus (HCMV) Glycoprotein B/gB; Polyclonal Antibody; Human cytomegalovirus (HCMV) Glycoprotein B/gB Antibody; Rabbit PAb; Antigen Affinity Purified; anti-gB antibody
Host
Rabbit
Reactivity
CMV
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CMV Glycoprotein B/gB
Purity/Purification
Protein A & Antigen Affinity
Form/Format
0.2um filtered solution in PBS
Concentration
1.05 mg/ml (varies by lot)
Applicable Applications for anti-gB antibody
ELISA (EIA)
Application Notes
Test Samples: Recombinant Protein ; Test Results: Pass
ELISA: 1:5000-1:10000
This antibody can be used at 1:5000-1:10000 with the appropriate secondary reagents to detect CMV gB.
Optimal concentrations/dilutions should be determined by the end user.
ELISA: 1:5000-1:10000
This antibody can be used at 1:5000-1:10000 with the appropriate secondary reagents to detect CMV gB.
Optimal concentrations/dilutions should be determined by the end user.
Immunogen
Recombinant Human CMV gB/Glycoprotein B protein
Preparation
Produced in rabbits immunized with purified, recombinant Human CMV gB extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907). Human cytomegalovirus gB specific IgG was purified by Human CMV gB affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles
Related Product Information for anti-gB antibody
Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
References
Lopper M, et al. (2002). Disulfide bond configuration of human cytomegalovirus glycoprotein B. J Virol. 76(12): 6073-82.Isaacson MK, et al. (2009) Human cytomegalovirus glycoprotein B is required for virus entry and cell-to-cell spread but not for virion attachment, assembly, or egress. J Virol. 83(8): 3891-903.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The gB (Catalog #AAA27727) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Human cytomegalovirus (HCMV) Glycoprotein B/gB Antibody, Rabbit PAb, Antigen Affinity Purified reacts with CMV and may cross-react with other species as described in the data sheet. AAA Biotech's cytomegalovirus (HCMV) Glycoprotein B/gB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Test Samples: Recombinant Protein ; Test Results: Pass ELISA: 1:5000-1:10000 This antibody can be used at 1:5000-1:10000 with the appropriate secondary reagents to detect CMV gB. Optimal concentrations/dilutions should be determined by the end user. Researchers should empirically determine the suitability of the gB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cytomegalovirus (HCMV) Glycoprotein B/gB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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