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Rabbit DCN/Decorin Polyclonal Antibody | anti-DCN antibody
DCN/Decorin Rabbit anti-Human Polyclonal Antibody
Gene Names
DCN; CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
Reactivity
Mouse, Rat, Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide affinity chromatography.
Synonyms
DCN/Decorin, Antibody; DCN/Decorin Rabbit anti-Human Polyclonal Antibody; IHC-plus DCN/Decorin Antibody; DCN; CSCD; Decorin proteoglycan; PG-S2; PG40; Proteoglycan core protein; SLRR1B; Small leucine-rich protein 1B; PGII; PGS2; Bone proteoglycan II; Decorin; DSPG2; anti-DCN antibody
Host
Rabbit
Reactivity
Mouse, Rat, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
DCN Antibody detects endogenous levels of total DCN.
Purity/Purification
Peptide affinity chromatography.
Form/Format
PBS, pH7.4, 0.02% Sodium Azide, 50% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DCN antibody
Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB)
Application Notes
IF: 1:100-1:500
IHC-P: 5ug/ml
Peptide-ELISA: 1:20000-1:40000
WB: 1:500-1:2000
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking.
IHC-P: 5ug/ml
Peptide-ELISA: 1:20000-1:40000
WB: 1:500-1:2000
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking.
Target
Human DCN/Decorin
Immunogen
A synthesized peptide derived from human DCN.
Conjugation
Unconjugated
Preparation and Storage
Upon receipt, store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistochemistry)
(DCN/Decorin Antibody-1:100 staining human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistchemistry)
(DCN/Decorin Antibody-Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(DCN/Decorin Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(DCN/Decorin Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(DCN/Decorin Antibody-Western blot analysis of mouse liver tissue lysates (30ug per lane) using DCN antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(DCN/Decorin Antibody-Western blot analysis of extracts of rat muscle using DCN antibody.)
ICC (Immunocytochemistry)
(DCN/Decorin Antibody-Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25 degree C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-DCN antibody
Decorin antibody is an unconjugated rabbit polyclonal antibody to Decorin (DCN) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
8,266 Da
NCBI Official Full Name
decorin isoform a preproprotein
NCBI Official Synonym Full Names
decorin
NCBI Official Symbol
DCN
NCBI Official Synonym Symbols
CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
NCBI Protein Information
decorin
UniProt Protein Name
Decorin
UniProt Gene Name
DCN
UniProt Synonym Gene Names
SLRR1B
UniProt Entry Name
PGS2_HUMAN
