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Rabbit DDX1 Polyclonal Antibody | anti-DDX1 antibody
Anti-DDX1 Antibody
Gene Names
DDX1; DBP-RB; UKVH5d
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
DDX1, Antibody; Anti-DDX1 Antibody; ATP-dependent RNA helicase DDX1; DEAD box protein 1; DEAD box protein retinoblastoma; DBP-RB; DDX1 ; anti-DDX1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
740
Applicable Applications for anti-DDX1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes
WB: 0.1-0.25ug/ml (Human, Mouse, Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Immunogen
E Coli-derived human DDX1 recombinant protein (Position: K562-F740).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
WB (Western Blot)
IHC (Immunohistchemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IF (Immunofluorescence)
FCM (Flow Cytometry)
Related Product Information for anti-DDX1 antibody
Description: Rabbit IgG polyclonal antibody for DDX1 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.
Background: ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene. It is mapped to 2p24.3. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.
Background: ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene. It is mapped to 2p24.3. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.
References
1. Chen HC, Lin WC, Tsay YG, Lee SC, Chang CJ (October 2002). "An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K". The Journal of Biological Chemistry. 277 (43): 40403-9.
2. Cho WJ, Yoon WJ, Moon CH, Cha SJ, Song H, Cho HR, Jang SJ, Chung DK, Jeong CS, Park JW (November 2002). "Molecular cloning of a novel chaperone-like protein induced by rhabdovirus infection with sequence similarity to the bacterial extracellular solute-binding protein family 5". The Journal of Biological Chemistry. 277 (44): 41489-96.
3. Li L, Li HS, Pauza CD, Bukrinsky M, Zhao RY (2006). "Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions". Cell Research. 15 (11-12): 923-34.
2. Cho WJ, Yoon WJ, Moon CH, Cha SJ, Song H, Cho HR, Jang SJ, Chung DK, Jeong CS, Park JW (November 2002). "Molecular cloning of a novel chaperone-like protein induced by rhabdovirus infection with sequence similarity to the bacterial extracellular solute-binding protein family 5". The Journal of Biological Chemistry. 277 (44): 41489-96.
3. Li L, Li HS, Pauza CD, Bukrinsky M, Zhao RY (2006). "Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions". Cell Research. 15 (11-12): 923-34.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ATP-dependent RNA helicase DDX1
NCBI Official Synonym Full Names
DEAD-box helicase 1
NCBI Official Symbol
DDX1
NCBI Official Synonym Symbols
DBP-RB; UKVH5d
NCBI Protein Information
ATP-dependent RNA helicase DDX1
UniProt Protein Name
ATP-dependent RNA helicase DDX1
UniProt Gene Name
DDX1
UniProt Synonym Gene Names
DBP-RB
UniProt Entry Name
DDX1_HUMAN
