Rabbit DLL4 Polyclonal Antibody | anti-DLL4 antibody

DLL4 Antibody

Cat. Num. AAA31168

• Gene Names: DLL4; AOS6; delta4; hdelta2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(88%)
• Applications: Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DLL4, Antibody; DLL4 Antibody; AOS6; Delta 4; delta 4 precursor; Delta ligand 4; delta ligand 4 precursor; Delta like 4; Delta like 4 homolog; Delta like 4 protein; Delta like canonical Notch ligand 4; Delta like protein 4; Delta-like 4 (Drosophila); Delta-like protein 4; Delta4; DLL 4; Dll4; DLL4_HUMAN; Drosophila Delta homolog 4; hdelta2; Homeobox protein DLL-4; MGC126344; Notch ligand delta 2; Notch ligand DLL4; Notch ligand DLL4 precursor; XDLL-4; anti-DLL4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(88%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DLL4 Antibody detects endogenous levels of total DLL4.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 685

• Applicable Applications for anti-DLL4 antibody: Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: IF: 1:100-1:500; ICC: 1:100-1:500; WB: 1:500-1:2000; IHC: 1:50-1:200; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human DLL4, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IF (Immunofluorescence)

(AAA31168 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31168 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistchemistry-Paraffin)

(AAA31168 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31168 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31168 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31168 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31168 at 1/100 staining Rat pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from HEK293 , using DLL4 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-DLL4 antibody

Function: Involved in the Notch signaling pathway as Notch ligand (PubMed:11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (PubMed:20616313). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate (PubMed:17728344).

Subcellular Location: Cell membrane>Single-pass type I membrane protein.

Tissue Specificity: Expressed in vascular endothelium.

Subunit Structure: Interacts with NOTCH4. Interacts (via N-terminal DSL and MNNL domains) with NOTCH1 (via EGF-like domains).

Similarity: The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.

NCBI and Uniprot Product Information

• NCBI GI #: 9506545
• NCBI GeneID: 54567
• NCBI Accession #: NP_061947.1
• NCBI GenBank Nucleotide #: NP_061947.1
• UniProt Accession #: Q9NR61
• Molecular Weight: Observed Molecular Weight: (Observed)74kD.; Predicted Molecular Weight: (Calculated)75kDa.
• NCBI Official Full Name: delta-like protein 4
• NCBI Official Synonym Full Names: delta like canonical Notch ligand 4
• NCBI Official Symbol: DLL4
• NCBI Official Synonym Symbols: AOS6; delta4; hdelta2
• NCBI Protein Information: delta-like protein 4
• UniProt Protein Name: Delta-like protein 4
• UniProt Gene Name: DLL4
• UniProt Synonym Gene Names: Delta4