Rabbit DYNLL1/PIN Polyclonal Antibody | anti-DYNLL1 antibody

Anti-DYNLL1/PIN Antibody

Cat. Num. AAA19276

• Gene Names: DYNLL1; LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: DYNLL1/PIN, Antibody; Anti-DYNLL1/PIN Antibody; DYNLL1; DLC1; DNCL1; DNCLC1; HDLC1; Dynein light chain 1, cytoplasmic; 8 kDa dynein light chain; DLC8; Dynein light chain LC8-type 1; Protein inhibitor of neuronal nitric oxide synthase; PIN; dynein light chain LC8-type 1; anti-DYNLL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for DYNLL1/PIN detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• Applicable Applications for anti-DYNLL1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml|Human, Mouse, Rat|; IHC-P: 2-5ug/ml|Human|; ICC/IF: 5ug/ml|Human|; FC/FACS/FCM: 1-3ug/1x106 cells|Human, Mouse, Rat|; Direct ELISA: 0.1-0.5ug/ml|Human|
• Immunogen: E Coli-derived human DYNLL1/PIN recombinant protein (Position: M1-G89).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 12. Flow Cytometry analysis of U937 cells using anti-DYNLL1/PIN antibody (AAA19276).Overlay histogram showing U937 cells stained with AAA19276 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DYNLL1/PIN Antibody (AAA19276, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry)

(Figure 11. Flow Cytometry analysis of NRK cells using anti-DYNLL1/PIN antibody (AAA19276).Overlay histogram showing NRK cells stained with AAA19276 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DYNLL1/PIN Antibody (AAA19276, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry)

(Figure 10. Flow Cytometry analysis of HEPA1-6 cells using anti-DYNLL1/PIN antibody (AAA19276).Overlay histogram showing HEPA1-6 cells stained with AAA19276 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DYNLL1/PIN Antibody (AAA19276, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence)

(Figure 9. IF analysis of DYNLL1/PIN using anti- DYNLL1/PIN antibody (AAA19276).DYNLL1/PIN was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- DYNLL1/PIN Antibody (AAA19276) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry)

(Figure 8. IHC analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (AAA19276).DYNLL1/PIN was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DYNLL1/PIN Antibody (AAA19276) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 7. IHC analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (AAA19276).DYNLL1/PIN was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DYNLL1/PIN Antibody (AAA19276) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry)

(Figure 6. IHC analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (AAA19276).DYNLL1/PIN was detected in paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DYNLL1/PIN Antibody (AAA19276) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 5. IHC analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (AAA19276).DYNLL1/PIN was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DYNLL1/PIN Antibody (AAA19276) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (AAA19276).DYNLL1/PIN was detected in paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DYNLL1/PIN Antibody (AAA19276) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 3. IHC analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (AAA19276).DYNLL1/PIN was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DYNLL1/PIN Antibody (AAA19276) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 2. IHC analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (AAA19276).DYNLL1/PIN was detected in paraffin-embedded section of human gallbladder adenocarcinoma lymphoid tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DYNLL1/PIN Antibody (AAA19276) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (AAA19276).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat testis tissue lysatesLane 2: rat brain tissue lysatesLane 3: mouse testis tissue lysatesLane 4: mouse brain tissue lysatesLane 5: human Mcf-7 whole cell lysatesLane 6: human A549 whole cell lysatesLane 7: human Caco-2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DYNLL1/PIN antigen affinity purified polyclonal antibody (Catalog # AAA19276) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for DYNLL1/PIN at approximately 12KD. The expected band size for DYNLL1/PIN is at 12KD.)

Related Product Information for anti-DYNLL1 antibody

Dynein light chain 1, cytoplasmic is a protein that in humans is encoded by the DYNLL1 gene. Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized.

References

1. Dick, T., Ray, K., Salz, H. K., Chia, W. Cytoplasmic dynein (ddlc1) mutations cause morphogenetic defects and apoptotic cell death in Drosophila melanogaster. Molec. Cell. Biol. 16: 1966-1977, 1996.
2. Fuhrmann, J. C., Kins, S., Rostaing, P., El Far, O., Kirsch, J., Sheng, M., Triller, A., Betz, H., Kneussel, M. Gephyrin interacts with dynein light chains 1 and 2, components of motor protein complexes. J. Neurosci. 22: 5393-5402, 2002.
3. He, Y. J., Meghani, K., Caron, M. -C., Yang, C., Ronato, D. A., Bian, J., Sharma, A., Moore, J., Niraj, J., Detappe, A., Doench, J. G., Legube, G., Root, D. E., D'Andrea, A. D., Drane, P., De, S., Konstantinopoulos, P. A., Masson, J. -Y., Chowdhury, D. DYNLL1 binds to MRE11 to limit DNA end resection in BRCA1-deficient cells. Nature 563: 522-526, 2018.

NCBI and Uniprot Product Information

• NCBI GI #: 83267866
• NCBI GeneID: 8655
• NCBI Accession #: NP_001032583.1
• NCBI GenBank Nucleotide #: NM_001037494.1
• UniProt Accession #: P63167; Q15701
• Molecular Weight: 10,366 Da
• NCBI Official Full Name: dynein light chain 1, cytoplasmic
• NCBI Official Synonym Full Names: dynein, light chain, LC8-type 1
• NCBI Official Symbol: DYNLL1
• NCBI Official Synonym Symbols: LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1
• NCBI Protein Information: dynein light chain 1, cytoplasmic; 8 kDa dynein light chain; cytoplasmic dynein light polypeptide; dynein, cytoplasmic, light polypeptide 1; protein inhibitor of neuronal nitric oxide synthase
• UniProt Protein Name: Dynein light chain 1, cytoplasmic
• UniProt Gene Name: DYNLL1
• UniProt Synonym Gene Names: DLC1; DNCL1; DNCLC1; HDLC1; DLC8; PIN
• UniProt Entry Name: DYL1_HUMAN