Rabbit EIF2S1 Polyclonal Antibody | anti-EIF2S1 antibody

EIF2S1 Antibody (N-term)

Cat. Num. AAA28744

• Gene Names: EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
• Reactivity: Mouse (Predicted Reactivity: Zebrafish, Bovine, Chicken, Drosophila, Pig, Rat, Yeast)
• Applications: Western Blot, ELISA, Immunofluorescence, Immunohistochemistry
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: EIF2S1, Antibody; EIF2S1 Antibody (N-term); Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2S1; EIF2A; anti-EIF2S1 antibody

• Host: Rabbit
• Reactivity: Mouse (Predicted Reactivity: Zebrafish, Bovine, Chicken, Drosophila, Pig, Rat, Yeast)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This EIF2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human EIF2S1.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 60-89
• Sequence Length: 315

• Applicable Applications for anti-EIF2S1 antibody: Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry-paraffin (IHC-p)
• Application Notes: WB~~1:1000; IF~~1:10~50; IHC-P~~1:10~50
• Function: Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:16289705, PubMed:38340717). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre- initiation complex (43S PIC) (PubMed:16289705). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex (PubMed:16289705). In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (PubMed:16289705). EIF2S1/eIF2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:19131336, PubMed:33384352, PubMed:38340717). EIF2S1/eIF2-alpha also acts as an activator of mitophagy in response to mitochondrial damage: phosphorylation by EIF2AK1/HRI promotes relocalization to the mitochondrial surface, thereby triggering PRKN-independent mitophagy (PubMed:38340717).
• Cellular location: Cellular Location Cytoplasm, Stress granule {ECO:0000250|UniProtKB:Q6ZWX6}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:P56286}. Mitochondrion. Note=Colocalizes with NANOS3 in the stress granules (By similarity). Relocalizes to the surface of mitochondria in response to mitochondrial damage and phosphorylation by EIF2AK1/HRI (PubMed:38340717). {ECO:0000250|UniProtKB:Q6ZWX6, ECO:0000269|PubMed:38340717}
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(EIF2S1 Antibody (N-term) (Cat. #AAA28744)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2S1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of EIF2S1 Antibody (N-term) (Cat#AAA28744) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot)

(EIF2S1 Antibody (N-term) (Cat. #AAA28744) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the EIF2S1 antibody detected the EIF2S1 protein (arrow).)

Related Product Information for anti-EIF2S1 antibody

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

References

Shiota, M., et al. Int. J. Oncol. 36(6):1521-1531(2010)
Kulkarni, A.P., et al. Indian J. Biochem. Biophys. 47(2):67-74(2010)
Wehner, K.A., et al. Mol. Cell. Biol. 30(8):2006-2016(2010)
Backes, S., et al. J. Gen. Virol. 91 (PT 2), 470-482 (2010) :
Sreejith, R.K., et al. Biochem. Biophys. Res. Commun. 390(2):273-279(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 4758256
• NCBI GeneID: 1965
• NCBI Accession #: NP_004085.1
• NCBI GenBank Nucleotide #: NM_004094.4
• UniProt Accession #: P05198
• NCBI Official Full Name: eukaryotic translation initiation factor 2 subunit 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• NCBI Official Symbol: EIF2S1
• NCBI Official Synonym Symbols: EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
• NCBI Protein Information: eukaryotic translation initiation factor 2 subunit 1
• UniProt Protein Name: Eukaryotic translation initiation factor 2 subunit 1
• UniProt Gene Name: EIF2S1
• UniProt Synonym Gene Names: EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha
• UniProt Entry Name: IF2A_HUMAN