FCM (Flow Cytometry)
(ENAM Antibody (C-term) (AAA28699) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human ENAM Polyclonal Antibody | anti-ENAM antibody
ENAM Antibody (C-term)
Gene Names
ENAM; ADAI; AI1C; AIH2
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ENAM, Antibody; ENAM Antibody (C-term); Enamelin; ENAM; anti-ENAM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This ENAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1097-1126 amino acids from the C-terminal region of human ENAM.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
1097-1126
Sequence Length
1142
Applicable Applications for anti-ENAM antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P), Flow Cytometry (FC)
Application Notes
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IHC-P~~1:50~100
FC~~1:10~50
Function
Involved in the mineralization and structural organization of enamel. Involved un the extension of enamel during the secretory stage of dental enamel formation.
Cellular Location
Secreted, extracellular space, extracellular matrix {ECO:0000250|UnitProtKB:O97939}
Tissue Location
Expressed in tooth particularly in odontoblast, ameloblast and cementoblast.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM (Flow Cytometry)
(ENAM Antibody (C-term) (AAA28699) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM (Flow Cytometry)
(ENAM Antibody (C-term) (AAA28699) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry)
(ENAM antibody(C-term) (AAA28699) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENAM antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(ENAM antibody(C-term) (AAA28699) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENAM antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ENAM Antibody (C-term) (AAA28699) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ENAM antibody detected the ENAM protein (arrow).)
WB (Western Blot)
(ENAM Antibody (C-term) (AAA28699) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ENAM antibody detected the ENAM protein (arrow).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
128785
NCBI Official Full Name
enamelin
NCBI Official Synonym Full Names
enamelin
NCBI Official Symbol
ENAM
NCBI Official Synonym Symbols
ADAI; AI1C; AIH2
NCBI Protein Information
enamelin
UniProt Protein Name
Enamelin
UniProt Gene Name
ENAM
UniProt Entry Name
ENAM_HUMAN
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Product Notes
The ENAM enam (Catalog #AAA28699) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1097-1126. The ENAM Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P), Flow Cytometry (FC). WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50. Researchers should empirically determine the suitability of the ENAM enam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENAM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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