IHC (Immunohistochemistry)
(IHC(P):Rat Spleen Tissue)
FOXP3 Polyclonal Antibody | anti-FOXP3 antibody
Anti-FOXP3 Antibody
Gene Names
FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Immunogen Affinity Purified
Synonyms
FOXP3, Antibody; Anti-FOXP3 Antibody; Forkhead box protein P3; AIID; DIETER; Forkhead box P3; foxp3; FOXP3_HUMAN; FOXP3delta7; Immune dysregulation polyendocrinopathy enteropathy X linked; Immunodeficiency polyendocrinopathy enteropathy X linked; IPEX; JM2; MGC141961; MGC141963; OTTHUMP00000025832; OTTHUMP00000025833; OTTHUMP00000226737; PIDX; SCURFIN; XPID; forkhead box P3; anti-FOXP3 antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized; Contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Applicable Applications for anti-FOXP3 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes
Immunohistochemistry (IHC) Paraffin -
Concentration: 0.5-1ug/ml
Tested Species: Hu, Ms, Rat
Antigen Retrieval: By Heat
Tested Species: In house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications not tested. Optimal dilutions should be determined by end users.
Concentration: 0.5-1ug/ml
Tested Species: Hu, Ms, Rat
Antigen Retrieval: By Heat
Tested Species: In house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications not tested. Optimal dilutions should be determined by end users.
Immunogen
E Coli-derived human FOXP3 recombinant protein (Position: H101-P431). Human FOXP3 shares 88% amino acid (aa) sequence identity with mouse FOXP3.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection System
We recommend HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (please inquire) for IHC(P).
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(IHC(P):Rat Spleen Tissue)
IHC (Immunohistochemistry)
(IHC(P):Rat Spleen Tissue)
IHC (Immunohistochemistry)
(IHC(P): Mouse Spleen Tissue)
IHC (Immunohistochemistry)
(IHC(P): Mouse Spleen Tissue)
IHC (Immunohistochemistry)
(IHC(P): Human Tonsil Tissue)
IHC (Immunohistochemistry)
(IHC(P): Human Tonsil Tissue)
Related Product Information for anti-FOXP3 antibody
FOXP3(forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome(specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.
References
1. Allan, S. E., Passerini, L., Bacchetta, R., Crellin, N., Dai, M., Orban, P. C., Ziegler, S. F., Roncarolo, M. G., Levings, M. K.The role of 2 FOXP3 isoforms in the generation of human CD4+ Tregs.J. Clin. Invest. 115: 3276-3284, 2005. 2. Bennett, C. L., Christie, J., Ramsdell, F., Brunkow, M. E., Ferguson, P. J., Whitesell, L., Kelly, T. E., Saulsbury, F. T., Chance, P. F., Ochs, H. D.The immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) is caused by mutations of FOXP3.Nature Genet. 27: 20-21, 2001. 3. Hong, J., Li, N., Zhang, X., Zheng, B., Zhang, J. Z.Induction of CD4+CD25+ regulatory T cells by copolymer-I through activation of transcription factor Foxp3.Proc. Nat. Acad. Sci. 102: 6449-6454, 2005.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
forkhead box protein P3 isoform b
NCBI Official Synonym Full Names
forkhead box P3
NCBI Official Symbol
FOXP3
NCBI Official Synonym Symbols
JM2; AIID; IPEX; PIDX; XPID; DIETER
NCBI Protein Information
forkhead box protein P3
UniProt Protein Name
Forkhead box protein P3
UniProt Gene Name
FOXP3
UniProt Synonym Gene Names
IPEX
UniProt Entry Name
FOXP3_HUMAN
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Product Notes
The FOXP3 foxp3 (Catalog #AAA11651) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-FOXP3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FOXP3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin. Immunohistochemistry (IHC) Paraffin - Concentration: 0.5-1ug/ml Tested Species: Hu, Ms, Rat Antigen Retrieval: By Heat Tested Species: In house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications not tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the FOXP3 foxp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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