WB (Western Blot)
(Validation Data Western blot analysis of extracts of various cell lines, using GAA antibody (AAA28197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s)
Rabbit GAA Polyclonal Antibody | anti-GAA antibody
GAA Polyclonal Antibody
Gene Names
GAA; LYAG
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Synonyms
GAA, Antibody; GAA Polyclonal Antibody; GAA, LYAG; glucosidase alpha, acid; anti-GAA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Applicable Applications for anti-GAA antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
Immunogen
A synthetic peptide corresponding to a sequence within aminoacids 350-450 of human GAA (NP_000143.2).
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
WB (Western Blot)
(Validation Data Western blot analysis of extracts of various cell lines, using GAA antibody (AAA28197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s)
WB (Western Blot)
(Validation Data Western blot analysis of extracts of various cell lines, using GAA antibody (AAA28197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s)
Related Product Information for anti-GAA antibody
This gene encodes lysosomal alpha-glucosidase, which is essential for thedegradation of glycogen to glucose in lysosomes. The encoded preproprotein isproteolytically processed to generate multiple intermediate forms and the matureform of the enzyme. Defects in this gene are the cause of glycogen storage diseaseII, also known as Pompe's disease, which is an autosomal recessive disorder with abroad clinical spectrum. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed MW: 105kDa
Calculated MW: 105kDa
Calculated MW: 105kDa
NCBI Official Full Name
lysosomal alpha-glucosidase preproprotein
NCBI Official Synonym Full Names
glucosidase, alpha; acid
NCBI Official Symbol
GAA
NCBI Official Synonym Symbols
LYAG
NCBI Protein Information
lysosomal alpha-glucosidase
UniProt Protein Name
Lysosomal alpha-glucosidase
UniProt Gene Name
GAA
UniProt Entry Name
LYAG_HUMAN
Similar Products
Product Notes
The GAA gaa (Catalog #AAA28197) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAA Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GAA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500 - 1:2000 IF: 1:50 - 1:200 IP: 1:50 - 1:200. Researchers should empirically determine the suitability of the GAA gaa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.