IHC (Immunohistochemistry)
(IHC image of AAA27004 diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Rabbit anti-Human GBA Polyclonal Antibody | anti-GBA antibody
GBA Antibody
Gene Names
GBA; GCB; GBA1; GLUC
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Synonyms
GBA, Antibody; GBA Antibody; Glucosylceramidase; Acid beta-glucosidase; Alglucerase; Beta-glucocerebrosidase; Beta-GC; D-glucosyl-N-acylsphingosine glucohydrolase; Imiglucerase; GBA; GC; GLUC; anti-GBA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Sequence Length
536
Applicable Applications for anti-GBA antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:5000
IHC: 1:500-1:1000
IHC: 1:500-1:1000
Immunogen
Recombinant Human Glucosylceramidase protein (215-343AA)
Immunogen Species
Homo sapiens (Human)
Research Area
Neuroscience
Target Names
GBA
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
IHC (Immunohistochemistry)
(IHC image of AAA27004 diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA27004 diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysateAll lanes: GBA antibody at 3.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60, 58, 30, 51, 55 KDaObserved band size: 51 KDa)
WB (Western Blot)
(Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysateAll lanes: GBA antibody at 3.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60, 58, 30, 51, 55 KDaObserved band size: 51 KDa)
Product Categories/Family for anti-GBA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
glucosylceramidase isoform 1
NCBI Official Synonym Full Names
glucosylceramidase beta
NCBI Official Symbol
GBA
NCBI Official Synonym Symbols
GCB; GBA1; GLUC
NCBI Protein Information
glucosylceramidase
UniProt Protein Name
Glucosylceramidase
UniProt Gene Name
GBA
UniProt Synonym Gene Names
GC; GLUC; Beta-GC
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Product Notes
The GBA gba (Catalog #AAA27004) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GBA Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GBA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:500-1:1000. Researchers should empirically determine the suitability of the GBA gba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GBA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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