Rabbit anti-Human, Rat GLI2 Polyclonal Antibody | anti-GLI2 antibody

Anti-GLI2 Antibody Picoband

Cat. Num. AAA19737

• Gene Names: GLI2; HPE9; PHS2; THP1; THP2
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: GLI2, Antibody; Anti-GLI2 Antibody Picoband; cAMP-dependent protein kinase type I-alpha regulatory subunit; GLI family zinc finger 2; Zinc finger protein GLI2; GLI family zinc finger protein 2; Tax helper protein; GLI2; THP; anti-GLI2 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Expressed in breast cancers. Isoform 1 and isoform 4 areExpressed in HTLV-1-infected T-cell lines. Isoform 1 and isoform 2 are stronglyExpressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weaklyExpressed in HTLV-1-infected T-cell lines.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Applicable Applications for anti-GLI2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml, Human, Rat; IHC(Paraffin-embedded Section): 2-5ug/ml, Human; ICC/IF: 5ug/ml, Human; FC/FACS (Fixed): 1-3ug/1x10⁶ cells, Human; ELISA: 0.1-0.5ug/ml
• Immunogen: E coli-derived human GLI2 recombinant protein (Position: A46-A1398).
• Subcellular Localization: Nucleus, Cytoplasm, Cilium
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
• Cross Reactivity: No cross-reactivity with other proteins.
• Protein Function: Functions as transcription regulator in the hedgehog (Hh) pathway. Functions as transcriptional activator. May also function as transcriptional repressor. Requires STK36 for full transcriptional activator activity. Required for normal embryonic development. Act as transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Bind to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1. Are involved in the smoothened (SHH) signaling pathway. Acts as a transcriptional repressor.
• Preparation and Storage: At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 6. Flow Cytometry analysis of U20S cells using anti-GLI2 antibody (AAA19737).Overlay histogram showing U20S cells stained with AAA19737 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLI2 Antibody (AAA19737, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence)

(Figure 5. IF analysis of GLI2 using anti-GLI2 antibody (AAA19737) and anti-Beta Tubulin antibody (M01857-3).GLI2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A549 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: rat brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (#AAA19737) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GLI2 at approximately 180-200 kDa. The expected band size for GLI2 is at 220 kDa.)

Related Product Information for anti-GLI2 antibody

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Categories/Family for anti-GLI2 antibody

Primary Antibodies; Cancer; Cell Cycle; DNA/RNA; Epigenetics and Nuclear Signaling; Kinases/Phosphatases; Nuclear Import/Export; Protein Trafficking; RNA Processing; Signal Transduction

References

1. Bertolacini, C. D. P., Ribeiro-Bicudo, L. A., Petrin, A., Richieri-Costa, A., Murray, J. C. Clinical findings in patients with GLI2 mutations--phenotypic variability. Clin. Genet. 81: 70-75, 2012.2. Matsumoto, N., Fujimoto, M., Kato, R., Niikawa, N. Assignment of the human GLI2 gene to 2q14 by fluorescence in situ hybridization. Genomics 36: 220-221, 1996.3. Roessler, E., Ermilov, A. N., Grange, D. K., Wang, A., Grachtchouk, M., Dlugosz, A. A., Muenke, M. A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Hum. Molec. Genet. 14: 2181-2188, 2005.

NCBI and Uniprot Product Information

• NCBI GI #: 215274258
• NCBI GeneID: 2736
• UniProt Accession #: P10070; O60252; O60253; O60254; O60255; Q15590; Q15591; Q4JHT4
• Molecular Weight: 86,482 Da
• NCBI Official Full Name: Zinc finger protein GLI2
• NCBI Official Synonym Full Names: GLI family zinc finger 2
• NCBI Official Symbol: GLI2
• NCBI Official Synonym Symbols: HPE9; PHS2; THP1; THP2
• NCBI Protein Information: zinc finger protein GLI2; oncogene GLI2; tax helper protein 1; tax helper protein 2; GLI-Kruppel family member GLI2; tax-responsive element-2 holding protein; glioma-associated oncogene family zinc finger 2; tax-responsive element-25-bp sequence binding p
• UniProt Protein Name: Zinc finger protein GLI2
• UniProt Gene Name: GLI2
• UniProt Entry Name: GLI2_HUMAN