Knockout Validation
(Knockout Validation: Lane 1: Wild-type HL60 cell lysate; Lane 2: GCA knockout HL60 cell lysate; Predicted MW: 24kd Observed MW: 27kd Primary Ab: 1ug/ml Rabbit Anti-Human GCA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Rabbit Grancalcin Polyclonal Antibody | anti-GCA antibody
Grancalcin (GCA) Polyclonal Antibody
Gene Names
GCA; GCL
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Grancalcin, Antibody; Grancalcin (GCA) Polyclonal Antibody; GCL; EF Hand Calcium Binding Protein; anti-GCA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GCA. It has been selected for its ability to recognize GCA in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
217
Applicable Applications for anti-GCA antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human, Rat
Organism Species
Human
Fragment
GCA (Met1~Val139)
Quality Control
Content: The quality control contains recombinant GCA (Met1~Val139) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HL60 cell lysate; Lane 2: GCA knockout HL60 cell lysate; Predicted MW: 24kd Observed MW: 27kd Primary Ab: 1ug/ml Rabbit Anti-Human GCA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HL60 cell lysate; Lane 2: GCA knockout HL60 cell lysate; Predicted MW: 24kd Observed MW: 27kd Primary Ab: 1ug/ml Rabbit Anti-Human GCA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Serum; Lane2: Human HL-60 Cells; Lane3: Mouse Sp2/0 Cells; Lane4: Rat Spleen Tissue.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Serum; Lane2: Human HL-60 Cells; Lane3: Mouse Sp2/0 Cells; Lane4: Rat Spleen Tissue.)
Product Categories/Family for anti-GCA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,010 Da
NCBI Official Full Name
grancalcin isoform b
NCBI Official Synonym Full Names
grancalcin
NCBI Official Symbol
GCA
NCBI Official Synonym Symbols
GCL
NCBI Protein Information
grancalcin
UniProt Protein Name
Grancalcin
UniProt Gene Name
GCA
UniProt Synonym Gene Names
GCL
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Product Notes
The GCA gca (Catalog #AAA21015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Grancalcin (GCA) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Grancalcin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GCA gca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Grancalcin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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