Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: GDA knockout Hela cell lysate; Predicted MW: 53,51,42kd Observed MW: 52kd Primary Ab: 1ug/ml Rabbit Anti-Human GDA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Rabbit Guanine Deaminase Polyclonal Antibody | anti-GDA antibody
Polyclonal Antibody to Guanine Deaminase (GDA)
Gene Names
GDA; GAH; CYPIN; GUANASE; NEDASIN
Reactivity
Human, Mouse, Bovine
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Guanine Deaminase, Antibody; Polyclonal Antibody to Guanine Deaminase (GDA); anti-GDA antibody
Host
Rabbit
Reactivity
Human, Mouse, Bovine
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GDA. It has beenselected for its ability to recognize GDA in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
0.91mg/ml (varies by lot)
Sequence Length
478
Applicable Applications for anti-GDA antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
GDA (Met1~Val454)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse, Bovine
Conjugate
No Conjugate
Immunogen
Recombinant GDA (Met1~Val454) expressed in E Coli.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: GDA knockout Hela cell lysate; Predicted MW: 53,51,42kd Observed MW: 52kd Primary Ab: 1ug/ml Rabbit Anti-Human GDA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: GDA knockout Hela cell lysate; Predicted MW: 53,51,42kd Observed MW: 52kd Primary Ab: 1ug/ml Rabbit Anti-Human GDA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Serum; Lane2: Human Hela Cells; Lane3: Mouse Brain Tissue; Lane4: Mouse Kidney Tissue; Lane5: Bovine Brain Tissue.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Serum; Lane2: Human Hela Cells; Lane3: Mouse Brain Tissue; Lane4: Mouse Kidney Tissue; Lane5: Bovine Brain Tissue.)
Product Categories/Family for anti-GDA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,837 Da
NCBI Official Full Name
guanine deaminase isoform a
NCBI Official Synonym Full Names
guanine deaminase
NCBI Official Symbol
GDA
NCBI Official Synonym Symbols
GAH; CYPIN; GUANASE; NEDASIN
NCBI Protein Information
guanine deaminase
UniProt Protein Name
Guanine deaminase
UniProt Gene Name
GDA
UniProt Synonym Gene Names
KIAA1258; Guanase; Guanine aminase; GAH
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Product Notes
The GDA gda (Catalog #AAA20868) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Guanine Deaminase (GDA) reacts with Human, Mouse, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Guanine Deaminase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the GDA gda for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Guanine Deaminase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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