Rabbit HBEGF Polyclonal Antibody | anti-HBEGF antibody

HBEGF Polyclonal Antibody

Cat. Num. AAA28122

• Gene Names: HBEGF; DTR; DTS; DTSF; HEGFL
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry
• Purity: Affinity purification
• Synonyms: HBEGF, Antibody; HBEGF Polyclonal Antibody; HBEGF; DTR; DTS; DTSF; HEGFL; anti-HBEGF antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence Length: 208

• Applicable Applications for anti-HBEGF antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500 - 1:2000; IF/ICC: 1:50-1:200
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human HBEGF (NP_001936.1)
• Species Reactivity / Cross Reactivity: Human, Mouse, Rat
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of PC-12 cells using HB-EGF Rabbit pAb (AAA28122) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using HB-EGF Rabbit pAb (AAA28122) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts of Rat kidney, using HB-EGF antibody (AAA28122) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using HBEGF antibody (AAA28122) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Related Product Information for anti-HBEGF antibody

Heparin-binding EGF-like growth factor (HB-EGF) is a 12-16 kDa member of the epidermal growth factor (EGF)family. It possesses an EGF-like domain, and a heparin-binding motif. Mature HB-EGF is a soluble peptide thatarises from proteolytic processing of the transmembrane form. Human HB -EGF shows 76% and 73% aasequence identity with rat and mouse HB-EGF, respectively. It is required for normal cardiac valve formationand normal heart function, promotes smooth muscle cell proliferation. It may be involved in macrophage-mediated cellular proliferation; it is mitogenic for fibroblasts, but not endothelial cells. HB-EGF classified as agroup 2 ErbB ligand based on its ability to activate both the EGF/ErbB1 and ErbB4 receptors. Activityassociated with ErbB4 binding appears to be limited to non -mitogenic actions, while EGFR binding inducesboth mitogenic and non-mitogenic activity.

Product Categories/Family for anti-HBEGF antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 544477
• NCBI GeneID: 1839
• UniProt Accession #: Q99075; B2R821
• Molecular Weight: Calculated MW: 23kDa; Observed MW: 23kDa
• NCBI Official Full Name: Proheparin-binding EGF-like growth factor
• NCBI Official Synonym Full Names: heparin-binding EGF-like growth factor
• NCBI Official Symbol: HBEGF
• NCBI Official Synonym Symbols: DTR; DTS; DTSF; HEGFL
• NCBI Protein Information: proheparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
• UniProt Protein Name: Proheparin-binding EGF-like growth factor
• UniProt Gene Name: HBEGF
• UniProt Synonym Gene Names: DTR; DTS; HEGFL; HB-EGF; HBEGF; DT-R
• UniProt Entry Name: HBEGF_HUMAN